Tools and techniques available in the lab

• DNA, RNA Isolation from complex communities
• PCR, rt-PCR, q-PCR
• tRFLP analysis
• Different cloning techniques and strategies
• DNA sequencing (Sanger, 454)
• Sequence analysis
• Phylogenetic analyses of microbial communities
• Probe development for FISH experiments
• Epifluorescence and 3D confocal laser scanning microscopy (CLSM)
• Isolation and further chemical analysis of signal molecules (AHL-type)
• Construction of gfp or rfp tagged sensor strains for functional analyses