An integrative system biological approach for a combined phenotypic, metabolic and proteomic characterisation of diabetes Type II in mouse models
T.Gröger (HMGU), S.Neschen (IEG, HMGU), H.Sarioglu (PROT, HMGU), M.Eschner (HMGU), S.Ly-Verdu (HMGU), R.Zimmermann (UR/HMGU)
- Fig.1 Diabetes Pilot Project
Within the framework of “Deutschen Zentrum für Diabetesforschunge.V.(DZD)“ a cooperation project between the Institute of Experimental Genetics/German Mouse Clinic, the Department of Protein Science and the Cooperation Group “Complex Molecular Systems” has been undertaken (Fig.1).
The primary objective is a phenotypic, proteomic and metabolomic integrative characterization of type 2 diabetes, focused on statistical data analysis collation of different mouse models. In order to develop different pre-diabetic and diabetic states in mice studied, mouse models are dietary triggered.
Comprehensive metabolic and proteomic profiling of the same sample tissue is undergoing and data from phenotypes and omic levels will be combined integratively (Fig. 2). Discovery of early biomarker is the ultimate aim of the project. The cooperation group is focused on metabolic fingerprinting and will apply different chromatographic and mass spectrometric methods to uncover differences in the metabolic profile.
- Fig.2 System biology levels approach. (*I.G.T.: Impaired Glucose Tolerance; I.R.: Insuline Resistance)
