Animal Model Generation
The ES cell culture lab, managed by Susanne Bourier provides ES cells, reagents, infrastructure and protocols for the generation of targeted mouse ES cells. The photo shows mouse ES cell colonies cultivated on a layer of embryonic feeder cells. The Microinjection team operates the microinjection and embryotransfer labs for the microinjection of ES cells into blastocysts, pronucleus injection and embryo transfer. In addition the team performs sperm freezing and in vitro fertilisation for the IDG strain archive and the import of new strains into the IDG barrier mouse facility. The photos demonstrate the microinjection of ES cells into blastocysts (middle) and the pronucleus injection of DNA into zygotes (right).
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| Mouse ES cells | Blastocyst injection | Pronucleus injection |
most important publications:
Meyer M, de Angelis MH, Wurst W, Kühn R. (2010). Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases. Proc Natl Acad Sci USA, 107, 15022-15026.
Steuber-Buchberger, P., Wurst, W. and Kühn, R. (2008). Simultaneous Cre-mediated conditional knockdown of two genes in mice. Genesis 46, 144 -151.
Delic, S., Streif, S. Deussing, J.M., Weber, P., Ueffing, M., Hölter, S.M., Wurst, W. and Kühn, R. (2008). Genetic Mouse Models for Behavioral Analysis through Transgenic RNAi Technology. Genes Brain Behav.7, 821 – 830.
Chu, Y., Senghaas, N., Köster, R.W., Wurst, W. and Kühn, R. (2008). Novel Caspase-Suicide Proteins for Tamoxifen-Inducible Apoptosis. Genesis, 46, 530- 536.
Hitz, C. Wurst, W. and Kühn, R. (2007). Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference. Nucleic Acids Res. 35, e90
Group and Teamleaders, from left to right:
Wolfgang Wurst, Jens Hansen, Ralf Kühn, Joel Schick. Absent:Thomas Floss