immunology screen

Head

Prof. Dr. med. Dirk H. Busch

phone : +49 (089) 4140 6191
fax: +49 (089) 4140 4139
E-Mail: dirk.busch@lrz.tum.de

Mailing adress

Institute of Medical Microbiology, Immunology and Hygiene
Technical University Munich
Trogerstr. 9
81675 Munich

Scientist

Dr. Thure Adler

phone: +49 (089) 3187-3283

fax: +49 (089) 3187-3500

E-Mail: thure.adler@helmholtz-muenchen.de

Homepage

http://www.mikrobio.med.tu-muenchen.de/project-dirk-busch/

Ultimate goal of the Immunology Screen in the German Mouse Clinic is to identify mouse mutant lines, which can serve as representative models for specific human immunological diseases (chronic inflammatory diseases, autoimmune diseases, immune-deficiencies, defects in protective immunity).

Primary Screen

Flow cytometry:

frequency of: T cells, cytotoxic T cells, B cells, B1 B cells, B2 B cells, gamma-delta T cells, granulocytes, NK cells

Bioplex:

levels of: IgG1, IgG2a, IgG2b, IgG3, IgA, IgM

ELISA:

anti-DNA antibodies, rheumatoid factor

Secondary Screen (3 animals per sex/per genotype)

Detailed flow cytometry analysis of the cellular composition of spleen, lymph nodes, bone marrow, thymus.

Tertiary Screen

Antigen-specific immune responses and protective immunity

(only for specific questions, test systems are still in the process of establishment and require a special animal experiment permit)

antigen-specific antibody response after immunization (Ag-specific ELISA)
antigen-specific CTL response after immunization (MHC-multimer staining)
in vivo differentiation of antigen-specific cell populations after immunization (intracellular cytokine staining)
establishment of antigen-specific protective immunity (Listeria monocytogenes)

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