institute of molecular immunology (imi)

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Research Group Kremmer

Core facility for generation of monoclonal antibodies and cell sorting

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Service Unit Monoclonal Antibodies

The Service Unit Monoclonal Antibodies was founded in order to provide access for scientists of Helmholtz-Zentrum München and the Munich universities to specific reagents. The task of our unit is to produce high-affinity antibodies in a short time and in high quality. The unit has increased its capacity to 500 projects per year and can even handle external orders. We also have ongoing projects with large biotech companies.
The spectrum of the reagents we produce ranges from antibodies against pesticides to antibodies against mouse cell surface antigens.

Our services include:

  • Advice on the design of antigen
  • Production of monoclonal antibodies
  • Isotype determination of the produced antibodies
  • Antibody production up to 100 mg
  • Purification and labeling of antibodies
  • Long-term storage of antibody-producing hybridomes
  • Advice on the detailed characterization of antibodies

 

The contact person for scientific questions is .

The contact person for cooperations with industry is ,
Patents & Technology Transfer Department of the Helmholtz Zentrum München.

 

Contact Address of the Service Unit Monoclonal Antibodies

Dr. med. Elisabeth Kremmer
Office:+49(0)89 / 7099 -321
Laboratory:+49(0)89 / 7099 -318
Fax:+49(0)89 / 7099 -300
E-mail:

back to top | Service Unit Monoclonal Antibodies | Cell Sorting Services | Contact Addresses | Staff | Publications | Dissertations

 

Cell Sorting Services

        to Cell Sorting Services Home:

The human body is composed of different cells specialised in diverse functions. Dysfunction of individual cell-types cause distinct diseases. Cell sorting gives the researcher the potential to enrich specific cell types from blood or tissue in order to search selectively, in the separated cells, for the source of the diseases and for treatment options.

 

The Cell Sorting Services of the Hämatologikum enables scientists of Helmholtz-Zentrum München and researchers from the Munich region to have cells sorted and to have special flow cytometry analyses conducted. For the sorts and analyses we use the high speed flow cytometer (MoFlo or FACSAria), which we physically alter to adapt to special biological problems.

Our services:

  • Sterile high-speed sorts
  • Development and establishment of new flow cytometric methods
  • Advise on the combination of  dyes
  • Multiple parameter analyses and sorts
  • Sorts of genetically modified  (S2) and infectious (L2) cells
  • Sorting of fagile cells
  • Sorts on microtiter plates, microscopic slides and any other medium
  • Simultaneous sorts of up to 4 fractions
  • Sorts of rare events
  • Single cell sorts, cloning

 

The scientific contact person and contact person for technical problems is .

 

 

Contact Address of the Cell Sorting Services

Contact details
Dr. Joachim W. Ellwart
Phone:+49(0)89 / 7099 -208
Fax:+49(0)89 / 7099 -277
E-mail:
Website:http://www.cell-sorter.de

back to top | Service Unit Monoclonal Antibodies | Cell Sorting Services | Contact Addresses | Staff | Publications | Dissertations

 

Staff

back to top | Service Unit Monoclonal Antibodies | Cell Sorting Services | Contact Addresses | Staff | Publications | Dissertations

 

Publications

back to top | Service Unit Monoclonal Antibodies | Cell Sorting Services | Contact Addresses | Staff | Publications | Dissertations

 

Dissertations

Franz, M.:
Durchflusszytometrische Untersuchungen von Lymphozytensubpopulationen bei gesunden und kranken Hunden.
Tierärztliche Fakultät, LMU München (1996)

Pfaffenrath, H.:
Charakterisierung monoklonaler Antikörper gegen Blutzellen der Regenbogenforelle (Oncorhynchus mykiss).
Tierärztliche Fakultät, LMU München (1998)

Prengel, I.:
Entwicklung und immunmorphologische Charakterisierung monoklonaler Antikörper gegen Proteine des Zebrabärblings (Danio rerio).
Tierärztliche Fakultät, LMU München (2000)

Malamid, A.:
Monoklonale Antikörper für die Analyse der Genexpression in neuronalen Geweben.
Medizinische Fakultät, LMU München (2005) 

back to top | Service Unit Monoclonal Antibodies | Cell Sorting Services | Contact Addresses | Staff | Publications | Dissertations