Daniela M. Padula, PhD
Phone: 0049-89-3187-4733
email: daniela.padula@helmholtz-muenchen.de
Pitchfork function in mouse development
In vertebrates Sonic hedgehog (Shh) signaling depends on primary cilia and failure of cilia formation and missregulation of Shh signaling lead to embryonic defects and a variety of human disease. We previously identified Pitchfork (Pifo) as a protein essential for cilia disassembly (Kinzel et al., Dev Cell 19, 66-77, 2010). Pifo is expressed in regions of high Shh signaling in the mouse embryo, is activated by Shh agonist and suppressed by Shh antagonist in cell culture, suggesting that Pifo is a Shh target gene and might function in the Shh signaling pathway. The aim of this project is to investigate if Pifo is a direct Shh target gene and if Pifo functions in the Shh pathway.
Academic and Research Activities
| Nov. 2002 – Sept. 2007 | Studies of Bioengineering at University of Applied Sciences Munich Biology, Molecular Biology, Tissue Engineering |
| Nov. 2007 – Sept. 2009 | Studies of Micro- and Nanotechnique at University of Applied Sciences Munich Biomicro- and Bionanotechnique, Micro- and Nanotechnique in Medicine and Biology |
| Sept. 2006 – Aug. 2007 | Chirurgische Klinik und Poliklinik Department of Experimental Surgery and Regenerative Medicine, Prof. Dr. med. Matthias Schieker, Dr. Denitsa Docheva in cooperation with the University of Applied Sciences Munich, Prof. Dr. Hauke Clausen-Schaumann Diploma Thesis: “Characterization of human mesenchymal stem cells by atomic force microscopy” |
| Jan. 2009 – Sept. 2009 | University of Applied Sciences Munich Department of Precision- and Micro-Engineering, Engineering Physics, Prof. Dr. Hauke Clausen-Schaumann in cooperation with the Experimental Surgery and Regenerative Medicine Master Thesis: “Matrix-triggered growth- and biophysical changes of prostate carcinoma cells and co-culture analysis with human mesenchymal derived stem cells” |
| Since Jan. 2010 | PhD student in the group of Dr. Heiko Lickert. Project title “Pitchfork function in mouse development”. |
Publications
[1] Effect of collagen I and fibronectin on the adhesion, elasticity and cytoskeletal organization of prostate cancer cells. Docheva D, Padula D, Schieker M, Clausen-Schaumann H. Biochem Biophys Res Commun. 2010 Nov 12;402(2):361-6. Epub 2010 Oct 12.
[2] Establishment of immortalized periodontal ligament progenitor cell line and its behavioural analysis on smooth and rough titanium surface. Docheva D, Padula D, Popov C, Weishaupt P, Prägert M, Miosge N, Hickel R, Böcker W, Clausen-Schaumann H, Schieker M. Eur Cell Mater. 2010 May 14;19:228-41.
[3] Researching into the cellular shape, volume and elasticity of mesenchymal stem cells, osteoblasts and osteosarcoma cells by atomic force microscopy. Docheva D, Padula D, Popov C, Mutschler W, Clausen-Schaumann H, Schieker M. J Cell Mol Med. 2008 Apr;12(2):537-52.
