protein expression and purification facility (pepf)

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Facilities available at the Institute for Structural Biology (STB)

Innova 44R shaker/incubator

Bacterial cultivation

3 Innova 44R shaker/incubators (New Brunswick Scientic)

Centrifugation

  • 2 Sorvall Evolution RC centrifuges (Thermo Scientific)
  • 2 Eppendorf 5810R centrifuges

 

 

      Homogenizer

      Cell lysis

      • Sonopuls HD2200 Sonicator (Bandelin)
      • Homogenizer (Microfluidics)
      Äkta Purifier with fixed columns

      Protein purification

      • 2 Äkta Purifiers (GE Healthcare)
      TDA 305 triple detector array

      Protein characterization

      • DLS DynaPro NanoStar (Wyatt Technology)
      • SLS TDA 305 triple detector array (Malvern-Viscotek)
      • ThermoFluor assay - Stratagene Mx3005P (Agilent Technologies)

      Spectroscopy

      • Peqlab Nanodrop 2000 (Thermo Scientific)
      • BioPhotometer plus (Eppendorf)
         
         

      Other equipment

      • C1000 Thermo Cycler (Bio-Rad)
      • MicroModulyo freeze dryer (Thermo Scientific)
      • Savant SPD111V SpeedVac Concentrator (Thermo Scientific)