protein expression and purification facility (pepf)

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Escherichia coli expression vectors

Vector Vector map Promoter Antibiotic resistance Tag(s) Protease cleavage site Expression host Origin of replication Source Description
pACYCDuet-1 map T7/lac Cam N-His BL21 (DE3) p15A Novagen expression vector; co-expression of 2 target genes
pACYC-11 map T7/lac Cam N-His TEV BL21 (DE3) p15A EMBL-AG expression vector
pACYC-13 map T7/lac Cam C-His TEV BL21 (DE3) p15A EMBL-AG expression vector
pACYC-31 map T7/lac Cam N-His
N-GST
TEV BL21 (DE3) p15A EMBL-AG expression vector
pASK75 tet Amp Strep BL21 Biometra expression vector
pBAD/His A map araBAD Amp N-His EK Top10
LMG194
pUC Invitrogen expression vector
pBAD/His B map araBAD Amp N-His EK Top10
LMG194
pUC Invitrogen expression vector
pBAD/His C map araBAD Amp N-His EK Top10
LMG194
pUC Invitrogen expression vector
pBAD/mcs map araBAD Amp N-His Top10
LMG194
pUC EMBL-AG expression vector
pBADM-11 map araBAD Amp N-His TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-20 map araBAD Amp N-TrxA
N-His
TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-20(+) map araBAD Amp N-TrxA
N-His
TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-30 map araBAD Amp N-His
N-GST
TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-30(+) map araBAD Amp N-His
N-GST
TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-40(+) map araBAD Amp N-MBP TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-41(+) map araBAD Amp N-His
N-MBP
TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-52 map araBAD Amp N-leaderless-DsbA
N-His
TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-52(+) map araBAD Amp N-leaderless-DsbA
N-His
TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-60 map araBAD Amp N-NusA
N-His
TEV Top10
LMG194
pUC EMBL-AG expression vector
pBADM-60(+) map araBAD Amp N-NusA
N-His
TEV Top10
LMG194
pUC EMBL-AG expression vector
pBAT4 map T7 Amp BL21 (DE3) pUC EMBL-MH expression vector
pBAT5 map T7 Amp BL21 (DE3) pUC EMBL-MH expression vector
pCal-n map T7/lac Amp CBP thrombin BL21 (DE3) ColE1 Stratagene expression vector
pCDF-1b map T7/lac Spec/Strep N-His EK BL21 (DE3) CDF Novagen expression vector
pCDF-11 map T7/lac Spec/Strep N-His TEV BL21 (DE3) CDF EMBL-AG expression vector
pCDF-13 map T7/lac Spec/Strep C-His TEV BL21 (DE3) CDF EMBL-AG expression vector
pCDFDuet-1 map T7/lac Spec/Strep N-His BL21 (DE3) CDF Novagen expression vector; co-expression of 2 target genes
pET-3a map T7 Amp BL21 (DE3) pBR322 Novagen expression vector
pET-3b map T7 Amp BL21 (DE3) pBR322 Novagen expression vector
pET-3c map T7 Amp BL21 (DE3) pBR322 Novagen expression vector
pET-3d map T7 Amp BL21 (DE3) pBR322 Novagen expression vector
pET-12a map T7 Amp leader sequence BL21 (DE3) pBR322 Novagen expression vector
pET-14b map T7 Amp N-His Thrombin BL21 (DE3) pBR322 Novagen expression vector
pET-15b map T7/lac Amp N-His Thrombin BL21 (DE3) pBR322 Novagen expression vector
pET-16b map T7/lac Amp N-His10 Factor Xa BL21 (DE3) pBR322 Novagen expression vector
pET-19b map T7/lac Amp N-His EK BL21 (DE3) pBR322 Novagen expression vector
pET-20b(+) map T7 Amp leader sequence
C-His
BL21 (DE3) pBR322 Novagen expression vector for secreted proteins
pET-21d(+) map T7/lac Amp C-His BL21 (DE3) pBR322 Novagen expression vector
pET-22b(+) map T7/lac Amp leader sequence
C-His
BL21 (DE3) pBR322 Novagen expression vector for secreted proteins
pET-24a(+) map T7/lac Kan C-His BL21 (DE3) pBR322 Novagen expression vector
pET-24d(+) map T7/lac Kan C-His BL21 (DE3) pBR322 Novagen expression vector
pET-28a(+) map T7/lac Kan N-His
C-His
Thrombin BL21 (DE3) pBR322 Novagen expression vector
pET-28c(+) map T7/lac Kan N-His
C-His
Thrombin BL21 (DE3) pBR322 Novagen expression vector
pET-32a(+) map T7/lac Amp N-TrxA
N-His
C-His
Thrombin
EK
AD494 (DE3)
Origami (DE3)
pBR322 Novagen expression vector for disulfite bond containing proteins
pET-32b(+) map T7/lac Amp N-TrxA
N-His
C-His
Thrombin
EK
AD494 (DE3)
Origami (DE3)
pBR322 Novagen expression vector for disulfite bond containing proteins
pET-32c(+) map T7/lac Amp N-TrxA
N-His
C-His
Thrombin
EK
AD494 (DE3)
Origami (DE3)
pBR322 Novagen expression vector for disulfite bond containing proteins
pET-39b(+) map T7/lac Kan N-DsbA
C-His
Thrombin
EK
BL21 (DE3) pBR322 Novagen expression vector for secreted proteins
pET-40b(+) map T7/lac Kan N-DsbC
N-His
C-His
Thrombin
EK
BL21 (DE3) pBR322 Novagen expression vector for secreted proteins
pETcoco-1 map T7/lac Cam N-His EK BL21 (DE3) oriS
oriV
Novagen expression vector; allows single and medium vector copy number
pETDuet-1 map T7/lac Amp N-His BL21 (DE3) pBR322 expression vector;  co-expression of 2 target genes
pET DsbA-1b map T7/lac Kan N-LL-DsbA
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d, leaderless DsbA for cytoplasmic expression
pET DsbC-1b map T7/lac Kan N-LL-DsbC
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d, leaderless DsbC for cytoplasmic expression
pET GB-1a map T7/lac Kan N-His
N-GB1
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET GB-1b map T7/lac Kan N-GB1
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET GST-1a map T7/lac Kan N-His
N-GST
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET GST-1b map T7/lac Kan N-GST
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET MBP-1a map T7/lac Kan N-His
N-MBP
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET MBP-1b map T7/lac Kan N-MBP
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET NusA-1a map T7/lac Kan N-His
N-NusA
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET NusA-1b map T7/lac Kan N-NusA
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET TrxA-1a map T7/lac Kan N-His
N-TrxA
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET TrxA-1b map T7/lac Kan N-TrxA
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET Z fl-1b map T7/lac Kan N-Z-tag (fl)
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET Z2-1a map T7/lac Kan N-His
N-Z2
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pET Z2-1b map T7/lac Kan N-Z2
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pETM-10 map T7/lac Kan N-His
C-His
BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pETM-11 map T7/lac Kan N-His TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pETM-12 map T7/lac Kan N-His Thrombin BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pETM-13 map T7/lac Kan C-His BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pETM-14 map T7/lac Kan N-His 3C BL21 (DE3) pBR322 EMBL-AG modified pET-24d
pETM-20 map T7/lac Amp N-TrxA
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-32
pETM-21 T7/lac Amp N-TrxA
N-His
Thrombin BL21 (DE3) pBR322 EMBL-GS modified pET-32
pETM-22 map T7/lac Kan N-TrxA
N-His
3C BL21 (DE3) pBR322 EMBL-AG modified pET-24d
pETM-30 map T7/lac Kan N-His
N-GST
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pETM-33 map T7/lac Kan N-His
N-GST
3C BL21 (DE3) pBR322 EMBL-AG modified pET-24d
pETM-40 map T7/lac Kan N-MBP TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pETM-41 map T7 Kan N-His
N-MBP
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-9d
pETM-43 map T7/lac Kan N-MBP 3C BL21 (DE3) pBR322 EMBL-AG modified pET-24d
pETM-44 map T7/lac Kan N-His
N-MBP
3C BL21 (DE3) pBR322 EMBL-AG modified pET-24d
pETM-50 map T7/lac Kan N-DsbA
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d; vector for periplasmic expression
pETM-51 T7/lac Kan N-DsbA
N-His
Thrombin BL21 (DE3) pBR322 EMBL-GS modified pET-24d; vector for periplasmic expression
pETM-52 map T7/lac Kan N-leaderless-DsbA
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d, leaderless DsbA for cytoplasmic expression
pETM-54 map T7/lac Kan N-DsbA
N-His
3C BL21 (DE3) pBR322 EMBL-AG modified pET-24d
pETM-55 map T7/lac Kan N-LL-DsbA
N-His
3C BL21 (DE3) pBR322 EMBL-AG modified pET-24d; leaderless DsbA for cytoplasmic expression
pETM-60 map T7/lac Kan N-NusA
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d
pETM-66 map T7/lac Kan N-NusA
N-His
3C BL21 (DE3) pBR322 EMBL-AG modified pET-24d
pETM-70 map T7/lac Kan N-CBP TEV BL21 (DE3) pBR322 EMBL-AG modified pET-24d
pETM-80 map T7/lac Kan N-DsbC
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-24d, vector for periplasmic expression
pETM-82 map T7 Kan N-LL-DsbC
N-His
TEV BL21 (DE3) pBR322 EMBL-GS modified pET-9d; leaderless DsbC for cytoplasmic expression
pETM-90 map T7/lac Amp N-FTR TEV BL21 (DE3) pBR322 EMBL-AM expression vector
pGAT map T7 Amp N-His
N-GST
Thrombin pUC EMBL-MH expression vector
pGAT2 map T7 Amp N-His
N-GST
Thrombin BL21 pUC EMBL-MH expression vector
pGEX-3X map tac Amp N-GST Factor Xa BL21 pBR322 GE-Healthcare expression vector
pGEX-4T-1 map tac Amp N-GST Thrombin BL21 pBR322 GE-Healthcare expression vector
pGEX-4T-2 map tac Amp N-GST Thrombin BL21 pBR322 GE-Healthcare expression vector
pGEX-4T-3 map tac Amp N-GST Thrombin BL21 pBR322 GE-Healthcare expression vector
pGEX-5X-1 map tac Amp N-GST Factor Xa BL21 pBR322 GE-Healthcare expression vector
pGEX-5X-2 map tac Amp N-GST Factor Xa BL21 pBR322 GE-Healthcare expression vector
pGEX-5X-3 map tac Amp N-GST Factor Xa BL21 pBR322 GE-Healthcare expression vector
pGEX-6P-1 map tac Amp N-GST 3C BL21 pBR322 GE-Healthcare expression vector
pGEX-6P-2 map tac Amp N-GST 3C BL21 pBR322 GE-Healthcare expression vector
pGEX-6P-3 map tac Amp N-GST 3C BL21 pBR322 GE-Healthcare expression vector
pGEXM7 map tac AMP N-GST BL21 pBR322 expression vector
pHAT map T7/lac Amp N-His BL21 (DE3) pUC EMBL-MH expression vector
pHAT2 map T7/lac Amp N-His BL21 (DE3) pUC EMBL-MH expression vector
pKK223-3 map tac Amp lacIq containing strain e.g. JM105 GE-Healthcare expression vector
pKK233-2 trc Amp GE-Healthcare expression vector
pMal-c2 map tac Amp N-MBP factor Xa TB1 pBR322 NEB expression vector
pMal-p2 map tac Amp leader sequence
N-MBP
factor Xa TB1 pBR322 NEB vector for expression in periplasm
pProEx HTa map trc Amp N-His TEV BL21 Life Technologies expression vector
pProEx HTb map trc Amp N-His TEV BL21 Life Technologies expression vector
pProEx HTc map trc Amp N-His TEV BL21 Life Technologies expression vector
pQE-16 map T5/lac Amp N-DHFR
C-His
M15 [pREP4] ColE1 Qiagen expression vector
pQE-30 map T5/lac Amp N-His M15 [pREP4] ColE1 Qiagen expression vector
pQE-31 map T5/lac Amp N-His M15 [pREP4] ColE1 Qiagen expression vector
pQE-32 map T5/lac Amp N-His M15 [pREP4] ColE1 Qiagen expression vector
pQE-60 map T5/lac Amp C-His M15 [pREP4] ColE1 Qiagen expression vector
pQE-70 map T5/lac Amp C-His M15 [pREP4] ColE1 Qiagen expression vector
pQE-80L map T5/lac Amp N-His M15 ColE1 Qiagen expression vector also expressing LacI
pQE-81L map T5/lac Amp N-His M15 ColE1 Qiagen expression vector also expressing LacI
pQE-82L map T5/lac Amp N-His M15 ColE1 Qiagen expression vector also expressing LacI
pRSET A map T7 Amp N-His EK BL21 (DE3) pLysS ColE1 Invitrogen expression vector
pRSET B map T7 Amp N-His EK BL21 (DE3) pLysS ColE1 Invitrogen expression vector
pRSET C map T7 Amp N-His EK BL21 (DE3) pLysS ColE1 Invitrogen expression vector
pRSF-1b map T7/lac Kan N-His TEV BL21 (DE3) RSF1030 Novagen expression vector
pRSF-11 map T7/lac Kan N-His TEV BL21 (DE3) RSF1030 EMBL-AG expression vector
pRSF-13 map T7/lac Kan C-His TEV BL21 (DE3) RSF1030 EMBL-AG expression vector
pRSFDuet-1 map T7/lac Kan N-His BL21 (DE3) RSF1030 Novagen expression vector; co-expression of 2 target genes
pTrcHis2 A map trc/lac Amp C-His BL21 pUC Invitrogen expression vector
pTrcHis2 B map trc/lac Amp C-His BL21 pUC Invitrogen expression vector
pTrcHis2 C map trc/lac Amp C-His BL21 pUC Invitrogen expression vector
pTrcHis2/LacZ map trc/lac Amp C-His BL21 pUC Invitrogen expression vector
pZA31-Luc map PLtetO-1 Cam DH5αZ1 p15A UniHD-HB expression vector; contains luciferase gene
pZE12-Luc map PLlacO-1 Amp DH5αZ1 ColE1 UniHD-HB expression vector; contains luciferase gene
pZE21-MCS-1 map PLtetO-1 Kan DH5αZ1 ColE1 UniHD-HB high copy number expression vector
pZS*24-MCS-1 map Plac/ara-1 Kan DH5αZ1 pSC*101 UniHD-HB low copy number expression vector

EMBL-AG = EMBL-made vector by Arie Geerlof
EMBL-AM = EMBL-made vector by Ario de Marco/Elisabetta Cassata
EMBL-GS = EMBL-made vector by Gunter Stier
EMBL-MH = EMBL-made vector by Marko Hyvönen
UniHD-HB = vector made by Prof. Herman Bujard,University of Heidelberg, Germany