protein expression and purification facility (pepf)

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Escherichia coli expression vectors

VectorVector mapPromoterAntibiotic resistanceTag(s)Protease cleavage siteExpression hostOrigin of replicationSourceDescription
pACYCDuet-1mapT7/lacCamN-HisBL21 (DE3)p15ANovagenexpression vector; co-expression of 2 target genes
pACYC-11mapT7/lacCamN-HisTEVBL21 (DE3)p15AEMBL-AGexpression vector
pACYC-13mapT7/lacCamC-HisTEVBL21 (DE3)p15AEMBL-AGexpression vector
pACYC-31mapT7/lacCamN-His
N-GST
TEVBL21 (DE3)p15AEMBL-AGexpression vector
pASK75tetAmpStrepBL21Biometraexpression vector
pBAD/His AmaparaBADAmpN-HisEKTop10
LMG194
pUCInvitrogenexpression vector
pBAD/His BmaparaBADAmpN-HisEKTop10
LMG194
pUCInvitrogenexpression vector
pBAD/His CmaparaBADAmpN-HisEKTop10
LMG194
pUCInvitrogenexpression vector
pBAD/mcsmaparaBADAmpN-HisTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-11maparaBADAmpN-HisTEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-20maparaBADAmpN-TrxA
N-His
TEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-20(+)maparaBADAmpN-TrxA
N-His
TEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-30maparaBADAmpN-His
N-GST
TEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-30(+)maparaBADAmpN-His
N-GST
TEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-40(+)maparaBADAmpN-MBPTEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-41(+)maparaBADAmpN-His
N-MBP
TEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-52maparaBADAmpN-leaderless-DsbA
N-His
TEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-52(+)maparaBADAmpN-leaderless-DsbA
N-His
TEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-60maparaBADAmpN-NusA
N-His
TEVTop10
LMG194
pUCEMBL-AGexpression vector
pBADM-60(+)maparaBADAmpN-NusA
N-His
TEVTop10
LMG194
pUCEMBL-AGexpression vector
pBAT4mapT7AmpBL21 (DE3)pUCEMBL-MHexpression vector
pBAT5mapT7AmpBL21 (DE3)pUCEMBL-MHexpression vector
pCal-nmapT7/lacAmpCBPthrombinBL21 (DE3)ColE1Stratageneexpression vector
pCDF-1bmapT7/lacSpec/StrepN-HisEKBL21 (DE3)CDFNovagenexpression vector
pCDF-11mapT7/lacSpec/StrepN-HisTEVBL21 (DE3)CDFEMBL-AGexpression vector
pCDF-13mapT7/lacSpec/StrepC-HisTEVBL21 (DE3)CDFEMBL-AGexpression vector
pCDFDuet-1mapT7/lacSpec/StrepN-HisBL21 (DE3)CDFNovagenexpression vector; co-expression of 2 target genes
pET-3amapT7AmpBL21 (DE3)pBR322Novagenexpression vector
pET-3bmapT7AmpBL21 (DE3)pBR322Novagenexpression vector
pET-3cmapT7AmpBL21 (DE3)pBR322Novagenexpression vector
pET-3dmapT7AmpBL21 (DE3)pBR322Novagenexpression vector
pET-12amapT7Ampleader sequenceBL21 (DE3)pBR322Novagenexpression vector
pET-14bmapT7AmpN-HisThrombinBL21 (DE3)pBR322Novagenexpression vector
pET-15bmapT7/lacAmpN-HisThrombinBL21 (DE3)pBR322Novagenexpression vector
pET-16bmapT7/lacAmpN-His10Factor XaBL21 (DE3)pBR322Novagenexpression vector
pET-19bmapT7/lacAmpN-HisEKBL21 (DE3)pBR322Novagenexpression vector
pET-20b(+)mapT7Ampleader sequence
C-His
BL21 (DE3)pBR322Novagenexpression vector for secreted proteins
pET-21d(+)mapT7/lacAmpC-HisBL21 (DE3)pBR322Novagenexpression vector
pET-22b(+)mapT7/lacAmpleader sequence
C-His
BL21 (DE3)pBR322Novagenexpression vector for secreted proteins
pET-24a(+)mapT7/lacKanC-HisBL21 (DE3)pBR322Novagenexpression vector
pET-24d(+)mapT7/lacKanC-HisBL21 (DE3)pBR322Novagenexpression vector
pET-28a(+)mapT7/lacKanN-His
C-His
ThrombinBL21 (DE3)pBR322Novagenexpression vector
pET-28c(+)mapT7/lacKanN-His
C-His
ThrombinBL21 (DE3)pBR322Novagenexpression vector
pET-32a(+)mapT7/lacAmpN-TrxA
N-His
C-His
Thrombin
EK
AD494 (DE3)
Origami (DE3)
pBR322Novagenexpression vector for disulfite bond containing proteins
pET-32b(+)mapT7/lacAmpN-TrxA
N-His
C-His
Thrombin
EK
AD494 (DE3)
Origami (DE3)
pBR322Novagenexpression vector for disulfite bond containing proteins
pET-32c(+)mapT7/lacAmpN-TrxA
N-His
C-His
Thrombin
EK
AD494 (DE3)
Origami (DE3)
pBR322Novagenexpression vector for disulfite bond containing proteins
pET-39b(+)mapT7/lacKanN-DsbA
C-His
Thrombin
EK
BL21 (DE3)pBR322Novagenexpression vector for secreted proteins
pET-40b(+)mapT7/lacKanN-DsbC
N-His
C-His
Thrombin
EK
BL21 (DE3)pBR322Novagenexpression vector for secreted proteins
pETcoco-1mapT7/lacCamN-HisEKBL21 (DE3)oriS
oriV
Novagenexpression vector; allows single and medium vector copy number
pETDuet-1mapT7/lacAmpN-HisBL21 (DE3)pBR322expression vector;  co-expression of 2 target genes
pET DsbA-1bmapT7/lacKanN-LL-DsbA
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d, leaderless DsbA for cytoplasmic expression
pET DsbC-1bmapT7/lacKanN-LL-DsbC
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d, leaderless DsbC for cytoplasmic expression
pET GB-1amapT7/lacKanN-His
N-GB1
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET GB-1bmapT7/lacKanN-GB1
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET GST-1amapT7/lacKanN-His
N-GST
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET GST-1bmapT7/lacKanN-GST
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET MBP-1amapT7/lacKanN-His
N-MBP
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET MBP-1bmapT7/lacKanN-MBP
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET NusA-1amapT7/lacKanN-His
N-NusA
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET NusA-1bmapT7/lacKanN-NusA
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET TrxA-1amapT7/lacKanN-His
N-TrxA
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET TrxA-1bmapT7/lacKanN-TrxA
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET Z fl-1bmapT7/lacKanN-Z-tag (fl)
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET Z2-1amapT7/lacKanN-His
N-Z2
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pET Z2-1bmapT7/lacKanN-Z2
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pETM-10mapT7/lacKanN-His
C-His
BL21 (DE3)pBR322EMBL-GSmodified pET-24d
pETM-11mapT7/lacKanN-HisTEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pETM-12mapT7/lacKanN-HisThrombinBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pETM-13mapT7/lacKanC-HisBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pETM-14mapT7/lacKanN-His3CBL21 (DE3)pBR322EMBL-AGmodified pET-24d
pETM-20mapT7/lacAmpN-TrxA
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-32
pETM-21T7/lacAmpN-TrxA
N-His
ThrombinBL21 (DE3)pBR322EMBL-GSmodified pET-32
pETM-22mapT7/lacKanN-TrxA
N-His
3CBL21 (DE3)pBR322EMBL-AGmodified pET-24d
pETM-30mapT7/lacKanN-His
N-GST
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pETM-33mapT7/lacKanN-His
N-GST
3CBL21 (DE3)pBR322EMBL-AGmodified pET-24d
pETM-40mapT7/lacKanN-MBPTEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pETM-41mapT7KanN-His
N-MBP
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-9d
pETM-43mapT7/lacKanN-MBP3CBL21 (DE3)pBR322EMBL-AGmodified pET-24d
pETM-44mapT7/lacKanN-His
N-MBP
3CBL21 (DE3)pBR322EMBL-AGmodified pET-24d
pETM-50mapT7/lacKanN-DsbA
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d; vector for periplasmic expression
pETM-51T7/lacKanN-DsbA
N-His
ThrombinBL21 (DE3)pBR322EMBL-GSmodified pET-24d; vector for periplasmic expression
pETM-52mapT7/lacKanN-leaderless-DsbA
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d, leaderless DsbA for cytoplasmic expression
pETM-54mapT7/lacKanN-DsbA
N-His
3CBL21 (DE3)pBR322EMBL-AGmodified pET-24d
pETM-55mapT7/lacKanN-LL-DsbA
N-His
3CBL21 (DE3)pBR322EMBL-AGmodified pET-24d; leaderless DsbA for cytoplasmic expression
pETM-60mapT7/lacKanN-NusA
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d
pETM-66mapT7/lacKanN-NusA
N-His
3CBL21 (DE3)pBR322EMBL-AGmodified pET-24d
pETM-70mapT7/lacKanN-CBPTEVBL21 (DE3)pBR322EMBL-AGmodified pET-24d
pETM-80mapT7/lacKanN-DsbC
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-24d, vector for periplasmic expression
pETM-82mapT7KanN-LL-DsbC
N-His
TEVBL21 (DE3)pBR322EMBL-GSmodified pET-9d; leaderless DsbC for cytoplasmic expression
pETM-90mapT7/lacAmpN-FTRTEVBL21 (DE3)pBR322EMBL-AMexpression vector
pGATmapT7AmpN-His
N-GST
ThrombinpUCEMBL-MHexpression vector
pGAT2mapT7AmpN-His
N-GST
ThrombinBL21pUCEMBL-MHexpression vector
pGEX-3XmaptacAmpN-GSTFactor XaBL21pBR322GE-Healthcareexpression vector
pGEX-4T-1maptacAmpN-GSTThrombinBL21pBR322GE-Healthcareexpression vector
pGEX-4T-2maptacAmpN-GSTThrombinBL21pBR322GE-Healthcareexpression vector
pGEX-4T-3maptacAmpN-GSTThrombinBL21pBR322GE-Healthcareexpression vector
pGEX-5X-1maptacAmpN-GSTFactor XaBL21pBR322GE-Healthcareexpression vector
pGEX-5X-2maptacAmpN-GSTFactor XaBL21pBR322GE-Healthcareexpression vector
pGEX-5X-3maptacAmpN-GSTFactor XaBL21pBR322GE-Healthcareexpression vector
pGEX-6P-1maptacAmpN-GST3CBL21pBR322GE-Healthcareexpression vector
pGEX-6P-2maptacAmpN-GST3CBL21pBR322GE-Healthcareexpression vector
pGEX-6P-3maptacAmpN-GST3CBL21pBR322GE-Healthcareexpression vector
pGEXM7maptacAMPN-GSTBL21pBR322expression vector
pHATmapT7/lacAmpN-HisBL21 (DE3)pUCEMBL-MHexpression vector
pHAT2mapT7/lacAmpN-HisBL21 (DE3)pUCEMBL-MHexpression vector
pKK223-3maptacAmplacIq containing strain e.g. JM105GE-Healthcareexpression vector
pKK233-2trcAmpGE-Healthcareexpression vector
pMal-c2maptacAmpN-MBPfactor XaTB1pBR322NEBexpression vector
pMal-p2maptacAmpleader sequence
N-MBP
factor XaTB1pBR322NEBvector for expression in periplasm
pProEx HTamaptrcAmpN-HisTEVBL21Life Technologiesexpression vector
pProEx HTbmaptrcAmpN-HisTEVBL21Life Technologiesexpression vector
pProEx HTcmaptrcAmpN-HisTEVBL21Life Technologiesexpression vector
pQE-16mapT5/lacAmpN-DHFR
C-His
M15 [pREP4]ColE1Qiagenexpression vector
pQE-30mapT5/lacAmpN-HisM15 [pREP4]ColE1Qiagenexpression vector
pQE-31mapT5/lacAmpN-HisM15 [pREP4]ColE1Qiagenexpression vector
pQE-32mapT5/lacAmpN-HisM15 [pREP4]ColE1Qiagenexpression vector
pQE-60mapT5/lacAmpC-HisM15 [pREP4]ColE1Qiagenexpression vector
pQE-70mapT5/lacAmpC-HisM15 [pREP4]ColE1Qiagenexpression vector
pQE-80LmapT5/lacAmpN-HisM15ColE1Qiagenexpression vector also expressing LacI
pQE-81LmapT5/lacAmpN-HisM15ColE1Qiagenexpression vector also expressing LacI
pQE-82LmapT5/lacAmpN-HisM15ColE1Qiagenexpression vector also expressing LacI
pRSET AmapT7AmpN-HisEKBL21 (DE3) pLysSColE1Invitrogenexpression vector
pRSET BmapT7AmpN-HisEKBL21 (DE3) pLysSColE1Invitrogenexpression vector
pRSET CmapT7AmpN-HisEKBL21 (DE3) pLysSColE1Invitrogenexpression vector
pRSF-1bmapT7/lacKanN-HisTEVBL21 (DE3)RSF1030Novagenexpression vector
pRSF-11mapT7/lacKanN-HisTEVBL21 (DE3)RSF1030EMBL-AGexpression vector
pRSF-13mapT7/lacKanC-HisTEVBL21 (DE3)RSF1030EMBL-AGexpression vector
pRSFDuet-1mapT7/lacKanN-HisBL21 (DE3)RSF1030Novagenexpression vector; co-expression of 2 target genes
pTrcHis2 Amaptrc/lacAmpC-HisBL21pUCInvitrogenexpression vector
pTrcHis2 Bmaptrc/lacAmpC-HisBL21pUCInvitrogenexpression vector
pTrcHis2 Cmaptrc/lacAmpC-HisBL21pUCInvitrogenexpression vector
pTrcHis2/LacZmaptrc/lacAmpC-HisBL21pUCInvitrogenexpression vector
pZA31-LucmapPLtetO-1CamDH5αZ1p15AUniHD-HBexpression vector; contains luciferase gene
pZE12-LucmapPLlacO-1AmpDH5αZ1ColE1UniHD-HBexpression vector; contains luciferase gene
pZE21-MCS-1mapPLtetO-1KanDH5αZ1ColE1UniHD-HBhigh copy number expression vector
pZS*24-MCS-1mapPlac/ara-1KanDH5αZ1pSC*101UniHD-HBlow copy number expression vector

EMBL-AG = EMBL-made vector by Arie Geerlof
EMBL-AM = EMBL-made vector by Ario de Marco/Elisabetta Cassata
EMBL-GS = EMBL-made vector by Gunter Stier
EMBL-MH = EMBL-made vector by Marko Hyvönen
UniHD-HB = vector made by Prof. Herman Bujard,University of Heidelberg, Germany