protein expression and purification facility (pepf)

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Cloning protocols

Preparation of oligo solutions
PCR experiments
Digest of insert DNA
Digest and dephosphorylation of vector DNA
Ligation of DNA fragments
Preparation of chemically competent cells
Transformation of plasmid DNA into chemically competent cells

Ligase independent cloning (LIC)
Ligase independent cloning (LIC) using pETM-13/LIC
Creating a construct with a C-terminal His6-tag

Contact

Dr. Arie Geerlof
Phone: +49 (0)89 3187 4707
Fax: +49 (0)89 3187 xxxx
E-mail: arie.geerlof@helmholtz-muenchen.de

Address

Helmholz Zentrum München
Institute of Structural Biology
Building 43b
Ingolstädter Landstraße 1
D-85764 München
Germany

STB

The PEPF is part of the Institute of Structural Biology

News

Recently we have established a small facility for the cultivation of insect cells. Here we offer the possibility to express recombinant proteins in Sf21 cells using the baculovirus expression system. For more information please contact Arie Geerlof (4707).