genome analysis center (gac)

Screen of amino acids, hexoses and lipids

Platform quantifies different metabolites using Biocrates AbsoluteIDQtm technology. Liquid handling is done on ML Star Hamilton robot. Assays are based on LC-MS separation and detection (Agilent HPLC, CTC autosampler and API 4000 MS/MS). Reference: Illig T, Gieger C, Zhai G, Romisch-Margl W, Wang-Sattler R, Prehn C, Altmaier E, Kastenmuller G, Kato BS, Mewes HW, Meitinger T, de Angelis MH, Kronenberg F, Soranzo N, Wichmann HE, Spector TD, Adamski J, Suhre K (2010) A genome-wide perspective of genetic variation in human metabolism. Nature Genetics 42(2): 137-141

Portfolio:

1.       Types of samples measured: plasma, serum, tissue, urine,
       cerebrospinal fluid (CSF), lung lavage.
2.       Amount of sample requested: 
       liquids: 50 µL minimum, preferably 100 µL, tissue: 50 mg minimum.
3.       Sample handling: Standard Operating Procedures (SOP)
       have to be followed for the sample preparation.
4.       Species tested: human, mouse, rat, rabbit, hamster,
       lamb, bovine and zebrafish.
5.       Throughput: up to 900 samples/week.
6.       Costs: vary upon amount of samples and are available on request.
7.       Sample tracking: LIMS.
8.       Data output: .csv, .xls
9.       Amount of analytes: for the list of 163 metabolites and CV-values
      (coefficient of variation) for the measurement of each metabolite
      see Tables 1 and 2.

 

Steroid screen

High-throughput steroid analyses can be performed for several human and murine steroids.
The method uses online solid phase extraction and HPLC-MS/MS (API 4000 Q Trap). Description of the method is provided in reference: Haller F, Prehn C, Adamski J.  Quantification of steroids in human and mouse plasma using online solid phase extraction coupled to liquid chromatography tandem mass spectrometry Nature Protocols 2010 10.1038/nprot.2010.22. The method has been validated for: linearity (including determination of LOD and LOQ), recovery, intra-day and inter-day variability as well as intermediate precision (between experimentators). SOPs have to be followed during the sample preparation

Portfolio:

1.       Types of samples measured: plasma
2.       Minimal amount of sample requested:
      50 µL for mouse plasma,100 µL for human plasma.
3.       SOPs have to be observed during the sample preparation
4.       The following steroids are quantified in human: D4-androstenedione,
       testosterone, progesterone, 17-hydroxy-progesterone, cortisol and cortisone.
5.       The following steroids are quantified in murine: 4-androstenedione,
       testosterone, corticosterone.
6.       Throughput: up to 400 samples/ week.
7.       Costs: vary upon amount of samples and are available on request.
8.       Sample tracking: LIMS.
9.       Data output: .csv, .xls

 

 

Screen of bile acids

Portfolio:
- Types of samples measured: plasma.
- Minimal amount of sample requested: 100 µl.
- Standard Operating Procedures (SOP) have to be followed for the sample preparation.
- The following bile acids can be quantified:
  cholic acid, ursodeoxycholic acid, deoxycholic acid, chenodeoxycholic acid, lithocholic acid,
  glycochenodeoxycholic acid, glycocholic acid, glycodeoxycholic acid,
  taurochenodeoxycholic acid, taurocholic acid, taurodeoxycholic acid and taurolithocholic acid.
- Throughput: up to 250 samples/ week.
- Costs: vary upon amount of samples and are available on request.
- Sample tracking: LIMS.
- Data output: .csv, .xls.

Analysis of plasma bile acids is performed using a robust and sensitive HPLC-MS/MS (API4000 Q Trap) method. The method has been validated for: linearity (including determination of LOD and LOQ), recovery, intra-day and inter-day variability as well as intermediate precision (between experimentators). SOPs have to be followed during the sample preparation.