Overview
About Next-Generation Sequencing
Next-Generation Sequencing (NGS) is a driving force between the basic and biomedical research due to its very high throughput at a reasonable cost. Several specific applications include: genome sequencing de novo, mutation discovery (e.g. medical resequencing or strain-to-train analyses), defining variability of genomes, metagenomics (survey of organisms present in complex samples), analyses of protein-DNA interactions and chromatin packaging (ChIP) and RNA analyses (noncoding RNAs, expression profiling).
In contrast to Sanger sequencing no subclonig of DNA fragments is required prior to NGS. Typical single readouts for NGS range from 30 to 400 bases with a huge fold coverage. In a week 100 Mb (Roche 454, pyrosequencing based) or 1300 Mb (Illumina, polymerase based sequencing by synthesis) are sequenced in a single run.