in vitro assays

Protein-Protein-Interaction assays

• AlphaScreen interaction assay (homogenous assay)
• DELFIA time-resolved fluorescence assay (ELISA based assay)
• Microscale thermophoresis (MST)
• Pull down assays (GST, StrepTagII, His, etc.)

Enzymatic assays

• Protease cleavage assay using Aminomethylcoumarin (AMC) substrates
• Kinase assays (AlphaScreen, TR-FRET (HTRF), ELISA, WB detection)
• Phosphatase assays (AlphaScreen, TR-FRET (HTRF))  

Protein-Compound-Interaction

• Microscale thermophoresis  

Cell-based assays

• High-Content-Screening (HCS) using Operetta System
• HTS/HCS screeening in Stem Cells
• Fluorescence-based reporter assays
• Cell viability assays
• Apoptosis assays