Biochemical assays

Biochemical assays

  • Protein-Protein Interaction assays
    • AlphaScreen interaction assay (homogenous assay)
    • TR-FRET (HTRF) interaction assay (homogenous assay)
    • DELFIA time-resolved fluorescence assay (ELISA based assay)
    • Microscale thermophoresis (MST)
    • Pull down assays (GST, StrepTagII, His, GFP-Trap, etc.)
  • Enzymatic assays
    • DUBs (AMC, IQF-diUb, AlphaScreen, etc.)
    • Protease cleavage assay (e.g. using AMC substrates)
    • Kinase assays (AlphaScreen, TR-FRET (HTRF), ELISA, WB detection)
    • Phosphatase assays (AlphaScreen, TR-FRET (HTRF))  
    • in vitro Ubiquitination assay
  • Protein-Compound Interactions
    • Microscale thermophoresis
  • Nucleic Acid-Compound Interactions
    • Microscale thermophoresis