Guidelines

  • Preferences:
Plate format:96-well or 384-well (384 is preferred)
Assay volume 96-well:100 µl – 200 µl
Assay volume 384-well:30 µl – 60 µl
Assay temperature range:           23°C – 37°C
Reagent stability:10 hours at RT or 4°C


  • Criteria for automation:
Plate variation (%CV max & min):< 20%
Signal window:> 2
Z’ factor (biochemical assay):0.5 - 1
Z’ factor (HCS assay):> 0.2
DMSO tolerance:> 1%
Other criteria:Existence of counter/secondary assays