Mitarbeiter

Dr. Kai Höfig
Senior Scientist

Telefon: +49 89 3187 1607
E-Mail
Geb./Raum: HAE/265

Projektbeschreibung

Mein Interesse gilt den Regulationsmechanismen von RBPs und RNAs in Immun- und MEF Zellen. Mein derzeitiger Fokus liegt auf der Analyse der E3 Ligasefunktion von Roquin.

Publikationen

  • Hoefig KP, Rath N, Heinz GA, Wolf C, Dameris J, Schepers A, Kremmer E, Ansel KM, Heissmeyer V. Eri1 degrades the stem-loop of oligouridylated histone mRNAs to induce replication-dependent decay. Nat Struct Mol Biol. 2013;20(1):73-81
  • Vogel KU, Edelmann SL, Jeltsch KM, Bertossi A, Heger K, Heinz GA, Zöller J, Warth SC, Hoefig KP, Lohs C, Neff F, Kremmer E, Schick J, Repsilber D, Geerlof A, Blum H, Wurst W, Heikenwälder M, Schmidt-Supprian M, Heissmeyer V. Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation. Immunity. 2013;38(4):655-668
  • Kreth S, Limbeck E; Ludwig C, Hinske LC, Schütz SV, Thon N, Hoefig KP, Egensperger R,•Kreth FW. In human glioblastomas transcript elongation by alternative polyadenylation and miRNA targeting is a potent mechanism of MGMT silencing. Acta Neuropathol. 2013;125(5):671-681
  • Zhu JY, Strehle M, Frohn A, Kremmer E, Hoefig KP, Meister G, Adler H. Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68. J Virol. 2010;84(19):10266-10275
  • Merz H, Kaehler C, Hoefig KP, Branke B, Uckert W, Nadrowitz R, Cerry-Reiterer S, Herrmann H, Feller AC, Valent P. Interleukin-9 (IL-9) and NPM-ALK each generate mast cell hyperplasia as single 'hit' and cooperate in producing a mastocytosis-like disease in mice. Oncotarget. 2010;1(2):104-119
  • Hanke M, Hoefig K, Merz H, Feller AC, Kausch I, Jocham D, Warnecke JM, Sczakiel G. A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer. Urol Oncol. 2010;28(6):655-661
  • Elke Glasmacher, Kai P Höfig, Katharina U Vogel, Nicola Rath, Lirui Du, Christine Wolf, Elisabeth Kremmer, Xiaozhong Wang, Vigo Heissmeyer, Nature Immunology, 2010, doi:10.1038/ni.1902, Roquin binds inducible costimulator mRNA and effectors of mRNA decay to induce microRNA-independent post-transcriptional repression [PubMed Abstract...]
  • Hoefig KP*, Roehle A*,  Repsilber D, Thorns C, Ziepert M, Wesche KO, Thiere M, Loeffler M, Klapper W, Pfreundschuh M, Matolcsky A, Bernd HW, Reiniger L, Merz H, Feller AC. MicroRNA signatures characterize diffuse large B-cell lymphomas and follicular lymphomas. Br J Haematol. 2008;142(5):732-744
  • Hoefig KP, Thorns C, Roehle A, Kaehler C, Wesche KO, Repsilber D, Branke B, Thiere M, Feller AC, Merz H. Unlocking pathology archives for microRNA-profiling. Anticancer Res. 2008;28(1A):119-123
  • Jarutat T, Nickels C, Frisch C, Stellmacher F, Hofig KP, Knappik A, Merz H. Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue. Biol Chem. 2007;388(6):651-658
  • Thorns C, Kalies K, Fischer U, Hofig K, Krokowski M, Feller AC, Merz H, Bernd HW. Significant high expression of CD23 in follicular lymphoma of the inguinal region. Histopathology. 2007;50(6):716-719
  • Hofig KP, Moller R, Donaldson L, Putterill J, Walter C. Towards male sterility in Pinus radiata-a stilbene synthase approach to genetically engineer nuclear male sterility. Plant Biotechnol J. 2006;4(3):333-343
  • Hofig KP, Moyle RL, Putterill J, Walter C. Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis. Planta. 2003;217(6):858-867.
    Walter C, Charity J, Grace L, Hoefig K, Moeller R, Wagner A. Gene technologies in Pinus radiata and Picea abies: tools for conifer biotechnology in the 21st century. Plant Cell Tissue Organ Cult. 2002;(1):3-12

Methodische Artikel

  • Hoefig KP, Heissmeyer V. Measuring microRNA expression in size-limited FACS-sorted and microdissected samples. Methods Mol Biol. 2010;667:47-63
  • Hoefig KP, Repsilber D, Branke B, Roehle A, Thorns C, Jarutat T, Lenfert E, Kaehler C, Feller AC, Merz H. New application for the LightCycler 480 System: qPCR-based microRNA-profiling. Biochemica. 2007; 4:7-9

Reviews

  • Hoefig KP, Heissmeyer V. Degradation of oligouridylated histone mRNAs: see UUUUU and goodbye. WIREs RNA 2014; 5(4):577-589
  • Hoefig KP, Heissmeyer V. MicroRNAs grow up in the immune system. Curr Opin Immunol. 2008;20(3):281-287
  • Walter C, Bishop-Hurley S, Charity J, Find J, Grace L, Hoefig K, Holland L, Moeller R, Moody J, Wagner A, Walden A. Genetic engineering of Pinus radiata and Picea abies, production of transgenic plants and gene expression studies. Progress in Biotechnology. 2001;(18):211-222

Curriculum Vitae

Seit 06/2007Postdoc im Labor von Prof. Dr. Vigo Heissmeyer, Abteilung für molekulare Immunregulation, Helmholtz Zentrum München
03/2006-05/2007Laborleiter Molekularbiologische Diagnostik am Universitätsklinikum Schleswig-Holstein (UKSH) Campus Lübeck, Abteilung Pathologie
05/2003-03/2006Postdoc am UKSH Campus Lübeck, Abteilung Pathologie
1999 – 2003PhD an der Universität von Auckland (Neuseeland)
Titel: Promoter analysis from four Pinus radiata male cone-specific genes in the model plant species Arabidopsis and tobacco
1997-1998Diplomarbeit am Max-Planck-Institut für Experimentelle Medizin, Abteilung Immunochemie
Titel: cDNA Isolierung und Analyse des Maus-NEFA Gens sowie dessen Nachweis in Maus F9-Zellen; Heterologe Genexpression des Maus-NEFA in Drosophila S2-Zellen
06/1994-10/1997Laborassistent am Max-Planck-Institut für Experimentelle Medizin, Abteilung Pharmazie/Immunchemie
1992-1998Studium der Biologie in Göttingen
Hauptfächer: Biochemie, Genetik, Organische Chemie
1990-1992Biologisch-Technischer Assistent