Element Speciation

Element Speciation

The group “Elements and Element Speciation” develops methods along our project demands and for future analytical challenges:

  • Elemental mapping: We use LA-ICP-MS for elemental mapping in different kinds of sample types, such as tissue samples, e.g. brain slices, or environmental samples like sediments from Yellowstone gush springs. Special challenges are analytical protocols for quantitative elemental mapping.
  • Element speciation:For speciation analysis several different coupling methods are developed and optimized based on LC-ICP-MS and LC-ICP-OES. The methods are widely applied in our research on neurodegenerative pathologies. A special focus lays on CE-ICP-MS methods in 2D-identification procedures, speciation of nanoparticles and especially for Iron Redox Speciation of Ferritin, Fe2+ and Fe3+, paralleled by GSH/GSSG differentiation, both helping to characterize oxidative stress and helping to explain steps of ferroptosis mechanism. See the video explanation of the method here.
  • Element determination in low-mass solid samples: We operate and develop element determination methods for solid samples with very low sample mass, unsuitable for digestion. For such samples, we use a modern ETV system hyphenated to ICP-OES with transient signal monitoring.
  • Quality control and reference materials: Aside, methods are developed and validated in the frame of our cooperation with the Institute for Reference Materials and Measurements of the European Joint Research Center“, for certification of a broad range of standard reference materials (CRM), for reference value determination within the German EQUAS system or within the workgroup “Analysis in biological materials” of the Deutsche Forschungsgemeinschaft.
Inductively Coupled Plasma Mass Spectrometer
Source: Helmholtz Zentrum München


Selected Publications:

Lucio, M., Barbir, R., Lovrenčić, M. V., Varžić, S. C., Ljubić, S., Duvnjak, L. S., ... & Vrček, I. V. (2020). Association between arsenic exposure and biomarkers of type 2 diabetes mellitus in a Croatian population: A comparative observational pilot study. Science of The Total Environment, 137575.

Violi, F., Solovyev, N., Vinceti, M., Mandrioli, J., Lucio, M., & Michalke, B. (2020). The study of levels from redox-active elements in cerebrospinal fluid of amyotrophic lateral sclerosis patients carrying disease-related gene mutations shows potential copper dyshomeostasis. Metallomics.

Michalke, B., Willkommen, D., & Venkataramani, V. (2019). Iron Redox Speciation Analysis Using Capillary Electrophoresis Coupled to Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS). Frontiers in Chemistry, 7, 136.

Lucio, M., Willkommen, D., Schroeter, M., Sigaroudi, A., Schmitt-Kopplin, P., & Michalke, B. (2019). Integrative Metabolomic and metallomic analysis in a case control cohort with Parkinson´ s disease. Frontiers in aging neuroscience, 11, 331.

Michalke, B., Kramer, M. F., & Brehler, R. (2018). Aluminium (Al) speciation in serum and urine after subcutaneous venom immunotherapy with Al as adjuvant. Journal of Trace Elements in Medicine and Biology, 49, 178-183.