TOPIC II "Aerosol Toxicology"

Adverse health effects of aerosols can ideally be assessed by direct exposures of biological model systems.  Therefore the topic group Aerosol Toxicology develops and adapts predictive cell culture model systems and toxicity measurements for exposures at the air-liquid-interface (ALI). ALI exposures consider aerosol particles as well as the gas phase of the aerosol which makes this kind of exposure highly realistic. In order to analyze specific responses of the cell culture systems classical cytotoxicity and genotoxicity assays are combined with comprehensive multi-omics analyses. Responses on transcriptome, proteome and metabolome levels are measured in cooperation with HMGU core facilities and other project partners. Simultaneous measurements of the chemical and physical aerosol composition enable to find new relations between the regulation of certain genes, proteins or metabolites and certain aerosol properties. In that way, substances should be identified that are responsible for certain cellular responses and thus could have high impact on the onset or aggravation of environmental diseases. The aim is to develop specific strategies for disease prevention by targeted avoidance of so far unknown risk factors.