Dr. Kai Höfig
Senior Scientist

Phone: +49 89 3187 1607
Building/Room: HAE/265

Project description

I am interested in regulatory mechnisms performed by RBPs and non-coding RNAs in an immunological setting. My current focus is on the E3 ligase function of Roquin.


Original Publications

  • Hoefig KP and Heissmeyer V. Degradation of oligouridylated histone mRNAs: see UUUUU and goodbye. Wiley Interdiscip. Rev. RNA. 2014; 5 (4):577-589
  • Hoefig KP, Rath N, Heinz GA, Wolf C, Dameris J, Schepers A, Kremmer E, Ansel KM, Heissmeyer V. Eri1 degrades the stem-loop of oligouridylated histone mRNAs to induce replication-dependent decay. Nat Struct Mol Biol. 2013;20(1):73-81
  • Vogel KU, Edelmann SL, Jeltsch KM, Bertossi A, Heger K, Heinz GA, Zöller J, Warth SC, Hoefig KP, Lohs C, Neff F, Kremmer E, Schick J, Repsilber D, Geerlof A, Blum H, Wurst W, Heikenwälder M, Schmidt-Supprian M, Heissmeyer V. Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation. Immunity. 2013;38(4):655-668
  • Kreth S, Limbeck E; Ludwig C, Hinske LC, Schütz SV, Thon N, Hoefig KP, Egensperger R,•Kreth FW. In human glioblastomas transcript elongation by alternative polyadenylation and miRNA targeting is a potent mechanism of MGMT silencing. Acta Neuropathol. 2013;125(5):671-681
  • Zhu JY, Strehle M, Frohn A, Kremmer E, Hoefig KP, Meister G, Adler H. Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68. J Virol. 2010;84(19):10266-10275
  • Merz H, Kaehler C, Hoefig KP, Branke B, Uckert W, Nadrowitz R, Cerry-Reiterer S, Herrmann H, Feller AC, Valent P. Interleukin-9 (IL-9) and NPM-ALK each generate mast cell hyperplasia as single 'hit' and cooperate in producing a mastocytosis-like disease in mice. Oncotarget. 2010;1(2):104-119
  • Hanke M, Hoefig K, Merz H, Feller AC, Kausch I, Jocham D, Warnecke JM, Sczakiel G. A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer. Urol Oncol. 2010;28(6):655-661
  • Elke Glasmacher, Kai P Höfig, Katharina U Vogel, Nicola Rath, Lirui Du, Christine Wolf, Elisabeth Kremmer, Xiaozhong Wang, Vigo Heissmeyer, Nature Immunology, 2010, doi:10.1038/ni.1902, Roquin binds inducible costimulator mRNA and effectors of mRNA decay to induce microRNA-independent post-transcriptional repression [PubMed Abstract...]
  • Hoefig KP*, Roehle A*,  Repsilber D, Thorns C, Ziepert M, Wesche KO, Thiere M, Loeffler M, Klapper W, Pfreundschuh M, Matolcsky A, Bernd HW, Reiniger L, Merz H, Feller AC. MicroRNA signatures characterize diffuse large B-cell lymphomas and follicular lymphomas. Br J Haematol. 2008;142(5):732-744
  • Hoefig KP, Thorns C, Roehle A, Kaehler C, Wesche KO, Repsilber D, Branke B, Thiere M, Feller AC, Merz H. Unlocking pathology archives for microRNA-profiling. Anticancer Res. 2008;28(1A):119-123
  • Jarutat T, Nickels C, Frisch C, Stellmacher F, Hofig KP, Knappik A, Merz H. Selection of vimentin-specific antibodies from the HuCAL phage display library by subtractive panning on formalin-fixed, paraffin-embedded tissue. Biol Chem. 2007;388(6):651-658
  • Thorns C, Kalies K, Fischer U, Hofig K, Krokowski M, Feller AC, Merz H, Bernd HW. Significant high expression of CD23 in follicular lymphoma of the inguinal region. Histopathology. 2007;50(6):716-719
  • Hofig KP, Moller R, Donaldson L, Putterill J, Walter C. Towards male sterility in Pinus radiata-a stilbene synthase approach to genetically engineer nuclear male sterility. Plant Biotechnol J. 2006;4(3):333-343
  • Hofig KP, Moyle RL, Putterill J, Walter C. Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis. Planta. 2003;217(6):858-867.
    Walter C, Charity J, Grace L, Hoefig K, Moeller R, Wagner A. Gene technologies in Pinus radiata and Picea abies: tools for conifer biotechnology in the 21st century. Plant Cell Tissue Organ Cult. 2002;(1):3-12


  • Hoefig KP, Heissmeyer V. Measuring microRNA expression in size-limited FACS-sorted and microdissected samples. Methods Mol Biol. 2010;667:47-63
  • Hoefig KP, Repsilber D, Branke B, Roehle A, Thorns C, Jarutat T, Lenfert E, Kaehler C, Feller AC, Merz H. New application for the LightCycler 480 System: qPCR-based microRNA-profiling. Biochemica. 2007; 4:7-9


  • Hoefig KP, Heissmeyer V. Degradation of oligouridylated histone mRNAs: see UUUUU and goodbye. WIREs RNA 2014; 5(4):577-589
  • Hoefig KP, Heissmeyer V. MicroRNAs grow up in the immune system. Curr Opin Immunol. 2008;20(3):281-287
  • Walter C, Bishop-Hurley S, Charity J, Find J, Grace L, Hoefig K, Holland L, Moeller R, Moody J, Wagner A, Walden A. Genetic engineering of Pinus radiata and Picea abies, production of transgenic plants and gene expression studies. Progress in Biotechnology. 2001;(18):211-222


Curriculum Vitae

Seit 06/2007Postdoc in the lab of Prof. Dr. Vigo Heissmeyer, Department of Molecular Immunregulation, Helmholtz Center Munich
03/2006-05/2007Head of the lab of molecular diagnostics, University hospital Schleswig-Holstein (UKSH) Campus Luebeck, Department of Pathology
05/2003-03/2006Postdoc at the UKSH Campus Luebeck, Department of Pathology
1999 – 2003PhD student at Auckland University (New Zealand)
Thesis title: Promoter analysis from four Pinus radiata male cone-specific genes in the model plant species Arabidopsis and tobacco.
1997-1998Diploma work at the Max-Planck-Institute for experimental medicine, Department of immunochemistry
Thesis title: cDNA isolation and analysis of the mouse NEFA gene and proof of its expression in mouse F9 cells; heterologous gene expression of the mouse NEFA in Drosophila S2 cells
06/1994-10/1997Lab assistant at the Max-Planck-Institute for experimental medicine, Department of pharmacy/immunochemistry
1992-1998Student at the Goettingen University (Germany)
Main subjects: biochemistry, genetics, organic chemistry
1990-1992Apprenticeship to become a research technician (BTA)