Induced Pluripotent Stem Cells

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Generation and working with human iPS cells

Source: Helmholtz Zentrum München

The Core Facility Induced Pluripotent Stem Cells (iPSC) serves as a source of knowledge and expertise for researchers interested in generating and working with human induced pluripotent stem cells and their differentiated progeny.
The Core Facility provides the scientific community with a variety of human iPS cell lines as well as the capability of establishing and characterizing custom-made iPS cell lines from patient biopsies, utilizing state-of-the-art reprogramming techniques.

Services and Expertise

  • Derivation of the dermal fibroblasts from the skin biopsy
  • Isolation of the peripheral blood mononuclear cells commonly called PBMCs from the blood sample
  • Derivation of the iPS cells from fibroblasts and PBMCs using footprint-free method like mRNA and episomal plasmid reprogramming
  • Characterisation of the derived cell lines by checking the pluripotency status, differentiation potency and testing quality control like e.g. genomic stability
  • Differentiation of iPSC into specialized cell types like NPC
  • Training (how to handle, maintain and manipulate iPSC) and project consulting
  • Genome editing using CRISPR/ Cas system
  • Shipment and Storage

to the Core Facility Induced Pluripotent Stem Cells website

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