Molecular Endocrinology

Lipids are pivotal to cell function, as they serve as nutrients (fatty acids), storage molecules (triglycerides), structural compounds (phospholipids and cholesterol) or as signaling molecules (steroids), to name only some of the multiple functions. Lipid homeostasis is thus important for the health of an organism.
Our group works to expand the knowledge on lipid-associated pathways to support the development of treatment of lipid-related diseases like diabetes, breast and prostate cancer, endometriosis, or cardiovascular diseases.
Our focus lies on the characterization of steroid and lipid metabolizing enzymes by analyzing tissue distributions, expression levels, substrate specificities, influences of endocrine disruptors or inhibitors on enzyme function, or the impact of DNA polymorphisms or gene knockout on gene functionality, to give some examples. Techniques applied comprise beside others cloning, protein expression in bacteria, yeast, insect and mammalian cells, purification of proteins, promoter analyses, enzymatic analyses, metabolomics (LC-MS/MS, GC-MS), PCR, Western blotting, immunocytochemistry, or immunohistochemistry.

Graphic by Jerzy Adamski