Microscopy

Profile

Our group focuses on developing technology and applications on the combination of optical and optoacoustic microscopy in order to deliver contrast and imaging abilities not available in optical microscopes today. While optical fluorescence microscopy is able to achieve sub-microns resolution even below the diffraction limit in living biological specimens, it is restricted by light scattering to superficial investigations at shallow depths. Optoacoustic microscopy offers absorption contrast, which complements fluorescence signals. Importantly it can be implemented to resolve the origin of ultrasound waves generated in response to short nanosecond-range light pulses propagating through tissue, essentially ‘listening’ to the molecules absorbing light. Acoustic signals scatters less than light in tissues and can enable high resolution imaging at larger depths. Our goal is to merge the advantages of optical and acoustical methods, achieving high resolution imaging at greater depths.

  • Hybrid optoacoustic and multiphoton imaging of a mouse ear and a zebrafish larva ex-vivo. 1. Mouse ear. The Raster-Scan Optoacoustic Mesoscopy (RSOM) scan of an extended region of the ear shows blood vessels of different sizes. A multi-frequency reconstruction (red color: full bandwidth, cyan color: high frequencies) enhances small vessels and features. The white box indicates the region of hybrid microscopy scans. Optoacoustic Microscopy (OM, red color) visualizes blood vessels, Second-Harmonic Generation Microscopy (SHG, blue color) reveals collagen in the extracellular matrix and Third-Harmonic Generation Microscopy (THG, green color) shows keratinocytes of the epidermis and hair follicles. A Brightfield Microscopy (BF) scan was performed for reference. 2. Zebrafish larva: The RSOM scan shows melanophores, eyes and inner organs of the entire specimen. The microscopy scans simultaneously visualize single melanocytes (OM), muscles (SHG) and connective tissue (THG) of the fish tail.
  • Multimodal optoacoustic and multiphoton microscopy (MPOM) of human carotid atheroma.
    Multimodal optoacoustic and multiphoton microscopy (MPOM) of human carotid atheroma. Coarse OAM scan and widefield BF observation enable ROI selection for subsequent MPOM imaging. Overlay of OAM (red), SHG (green), THG (blue), and TPEF (white) demonstrates an unscathed condition of the connective tissue (e.g. collagen and elastin), no large inclusions or fissures, and a negligible amount of embedded blood residues at the shoulder region. Analogous examination indicates a coarse alternating pattern of moieties as well as a slightly disturbed morphology at the cap region, whereas a fine interleaving structure along with an advanced degradation of cell morphology is revealed at the lipid core. Image courtesy of Markus Seeger and Angelos Karlas. M. Seeger, et al., Photoacoustics, 2016.

Group leader

Image Name Job Title E-mail Telephone Building/Room
Dr. Chmyrov,
Andriy
Group Leader E-mail +49 (0) 89 3187 2140 56/039

Relevant publications

Markus Seeger, Angelos Karlas, Dominik Soliman, Jaroslav Pelisek, Vasilis Ntziachristos, Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma. Photoacoustics 4(3), 102–111 (2016).

D. Soliman, G. J. Tserevelakis, M. Omar, V. Ntziachristos, Combining microscopy with mesoscopy using optical and optoacoustic label-free modes. Scientific Reports 5, 12902 (2015).
 
George J. Tserevelakis, Dominik Soliman, et al. Hybrid multiphoton and optoacoustic microscope. Optics letters 39.7 (2014): 1819-1822.

People

Image Name Job Title E-mail Telephone Building/Room
Dr. Chmyrov,
Andriy
Group Leader E-mail +49 (0) 89 3187 2140 56/039
Dr. Pleitez,
Miguel Angel Rafael
Postdoctoral Fellow E-mail +49 (0) 89 4140 9018 Einsteinstr. 25, TranslaTUM (bldg. 522), room 22.3.36
Dr. Soldà,
Alice
Postdoctoral fellow E-mail +49 (0) 89 4140 9018 Einsteinstr. 25, TranslaTUM (bldg. 522), room 22.3.36
Dr. Uluc,
Nasire
Postdoctoral fellow E-mail Einsteinstr. 25, TranslaTUM (bldg. 522), room 22.3.36
Dr. Huang,
Yu-Shan
Postdoctoral fellow E-mail +49 (0) 89 3187-49340 43b/102
Seeger,
Markus
Ph.D. Student E-mail +49 (0) 89 4140 9172 Einsteinstr. 25, TranslaTUM (bldg. 522), room 22.3.32
Yuan,
Tao
Ph.D. Student E-mail Einsteinstr. 25, TranslaTUM (bldg. 522), room 22.3.32
Huang,
Yuanhui
Ph.D. Student E-mail +49 (0) 89 3187 3603 56/057
Schätz,
Benedikt
Internship E-mail +49 (0) 89 3187 49204 Einsteinstr. 25, TranslaTUM (bldg. 522), room 22.3.32
Visscher,
Mirjam
Internship Einsteinstr. 25, TranslaTUM (bldg. 522), room 22.3.42
Gasparin,
Francesca
Ph.D. Student Einsteinstr. 25, TranslaTUM (bldg. 522), room 22.3.42

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