Research

• Computational modelling of stem cell fates based on single-cell gene expression data
  • Structured Gaussian Process Latent Variable model
  • scLVM - Dissecting heterogeneities in single-cell RNA-seq data
• Bayesian gene set enrichment of multi-omics data
• Image processing of time-lapse microscopy movies
• Modelling outcomes of radiotherapy treatments

Publications

• Buettner F*, Natarajan KN*, Casale FP, Proserpio V, Scialdone A, Theis FJ, Teichmann SA, Marioni JC & Stegle O, 2015. Accounting for cell-to-cell heterogeneity in single-cell RNA-Seq data reveals novel structure between cells, Nature Biotechnology, in the press.  *equal contribution
• Sass S, Buettner F, Mueller NS & Theis FJ 2014. RAMONA: a web application for multilevel ontology analysis, Bioinformatics, btu610.
• Schmidt AM, Fagerer SR, Jefimovs K, Buettner F, Marro C, Siringil E, Boehlen K, Pabst M & Ibanez AJ 2014. Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level. Analyst, 139(22), 5710-5718.
• Winkler C, Krumsiek J, Buettner F, Angermüller C, Giannopoulou EZ, Theis FJ, Ziegler AG, Bonifacio E 2014. Feature Ranking of Type 1 Diabetes Susceptibility Genes Improves Prediction of Type 1 Diabetes, Diabetologia, 57(12), 2521-2529.
• Buettner F, Moignard V, Göttgens B & Theis FJ 2014. Probabilistic PCA of censored data: accounting for uncertainties in the visualisation of high-throughput single-cell qPCR data, Bioinformatics, btu134
• Ebert MA, Gulliford SL, Buettner F, Foo K, Haworth A, Kennedy A, Joseph DJ & Denham JW 2014. Two non-parametric methods for derivation of constraints from radiotherapy dose-histogram data, Phys Med Biol 59(13), N101-N111
• Sass S*, Buettner F*, Müller NS, Theis FJ. A modular framework for gene set analysis integrating multilevel omics data. Nucleic Acids Res. 2013 Aug 23 *equal contribution
• Moignard V, Macaulay IC, Swiers G, Buettner F, Schütte J, Calero-Nieto FJ, Kinston S, Joshi A, Hannah R, Theis FJ, Jacobsen SE, Bruijn MFd, Göttgens B 2013 Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis. Nature Cell Biology, 15(4):363-72
• Buettner F & Theis F J 2012 A novel approach for resolving differences in single-cell gene expression patterns from zygote to blastocyst. Bioinformatics 28(18):i626-i632
• Buettner F, Gulliford S L, Webb S, Sydes M R, Dearnaley D P & Partridge M 2012 The dose-response of the anal sphincter region--an analysis of data from the MRC RT01 trial. Radiother Oncol 103(3),347-52
• Buettner F, Miah AM , Gulliford S L, Hall E, Harrington KJ , Webb S, Partridge M & Nutting C M 2012 Novel approaches to improve the therapeutic index of head and neck radiotherapy: an analysis of data from the PARSPORT randomised phase III trial. Radiother Oncol 103(1),82-7
• Buettner F, Gulliford S L, Webb S & Partridge M 2011 Modeling late rectal toxicities based on a parameterized representation of the 3D dose distribution. Phys Med Biol, 56(7)  2103-19
• Buettner F, Gulliford S L, Webb S & Partridge M 2010 Using Bayesian logistic regression to evaluate a new type of dosimetric constraint for prostate radiotherapy treatment planning Med Phys 37(4), 1768-1777.
• Buettner F, Gulliford S L, Webb S, Sydes M R, Dearnaley D P & Partridge M 2009b Assessing cor- relations between the spatial distribution of the dose to the rectal wall and late rectal toxicity after prostate radiotherapy: an analysis of data from the MRC RT01 trial (ISRCTN 47772397). Phys Med Biol 54(21), 6535-6548.
• Buettner F, Gulliford S L, Webb S & Partridge M 2009a Using dose-surface maps to predict radiation- induced rectal bleeding: a neural network approach. Phys Med Biol 54(17), 5139-5153.
• Seco J, Sharp G C, Wu Z, Gierga D, Buettner F & Paganetti H 2008 Dosimetric impact of motion in free-breathing and gated lung radiotherapy: A 4D Monte Carlo study of intrafraction and interfraction effects Med Phys 35(1), 356-366.
• Volpe G, Singh GP, Buettner F, Morales A, Petrov D, 2006 Optical Trapping Dynamics for Cell Identification in Proc. SPIE 6326
• Buettner F, Gulliford S L, Partridge M, Sydes M R, Dearnaley D P & Webb S 2009 Assessing correla- tions between the spatial distribution of dose to the rectal wall and late rectal toxicity after prostate radiotherapy in Proc. World Congress of Medical Physics and Biomedical Engineering, p. 908-911.
• Buettner F, Gulliford SL, Webb S & Partridge M 2009 Using Bayesian logistic regression with high- order interactions to model radiation-induced toxicities following radiotherapy in Proc. 8th Int. Conf. on Machine Learning and Applications, p. 451-456.
• Buettner F, Gulliford SL, Webb S & Partridge M 2010 Using a parameterized representation of the 3D dose distribution to predict radiation-induced toxicities in Proc. XVIth Conf. on the use of Computers in Radiotherapy.
• Buettner F, Miah A B, Gulliford S L, Harrington J K, Webb S, Nutting C M & Partridge M 2010 Using a Bayesian Feature Selection Algorithm to Identify Dose-response Models Based on the Shape of the 3D Dose-distribution: An example from a head and neck trial in Proc. 9th Int. Conf. on Machine Learning and Applications, p. 740-45.

Curriculum Vitae