Genetic Tools Development

Animal Model Generation

The ES cell culture lab, managed by Susanne Bourier provides ES cells, reagents, infrastructure and protocols for the generation of targeted mouse ES cells. The photo shows mouse ES cell colonies cultivated on a layer of embryonic feeder cells. The Microinjection team operates the microinjection and embryotransfer labs for the microinjection of ES cells into blastocysts, pronucleus injection and embryo transfer. In addition the team performs sperm freezing and in vitro fertilisation for the IDG strain archive and the import of new strains into the IDG barrier mouse facility. The photos demonstrate the microinjection of ES cells into blastocysts (middle) and the pronucleus injection of DNA into zygotes (right).


      

Mouse ES cells                             Blastocyst injection                   Pronucleus injection