Research

SCADEV - The Stem Cell-based Assay Development Platfrom

Human pluripotent stem cells can be propagated in culture, as they self-renew and are able to differentiate into all derivatives of the three germ layers. Owing to these properties and their close physiological similarities to cells in situ, human pluripotent stem cells hold great promises as a model system for studying human development and disease, as a source of specialized cells for regenerative medicine, and as a ‘bioassay’ for early toxicity and efficacy assessment during drug development. Based on our long-lasting expertise1,2, we have established a platform at HMGU in June 2014, the Stem-Cell Assay DEVelopment (SCADEV) Platform, for assays development using human pluripotent stem cells and their derivatives and have developed robust new protocols for their differentiation to relevant cell types. The main applications for these assays are high-throughput and high-content screening for drug discovery as well as cytotoxicity testing. The platform also offers additional services and develops new Stem Cell products. 

Our services:

  • Testing of cytotoxicity effects of verified hits & lead compounds on cardiomyocytes, hepatocytes and neurons derived from human pluripotent stem cells.
  • Adaptation of human pluripotent stem cells or their derivatives to high-content and high-throughput screening for drug discovery

Our products:

  • Cardiomyocytes, hepatocytes, neurons, and other cell types on demand derived from human pluripotent stem cells (NIH approved cell lines and cell lines derived from healthy patient through reprogramming done in the Induced Pluripotent Stem Cells Core Facility link to Micha Drukker’s Unit (https://www.helmholtz-muenchen.de/ipsc/index.html#!prettyPhoto)
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  • gDNA, totalRNA, cDNA, proteins extracted from human pluripotent stem cells and their derivatives
  • Solubilized basement membranes for the efficient maintenance and differentiation of human pluripotent stem cells (in progress)

 Movie: hPSC-derived cardiomyoctes (~ 30MB)

 

 

hPSC-derived hepatocytes stained with Actin antibody
hPSC-derived neurons stained with bet3-Tubulin antibody

Contact: SCADEV technician:

References:
1. Desbordes, S.C.Studer, L. “Adapting human pluripotent stem cells to high-throughput and high-content screening”. Nature Protocols 2013 Jan;8(1):111-30. # Corresponding author
2. Desbordes, S.C. #, Placantonakis, D.G., Ciro, A., Socci, N.D., Lee, G., Djaballah, H. and Studer, L># “High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells”. Cell Stem Cell 2008 Jun 5;2(6):602-12. #Co-corresponding authors