Team Member

Johanna Siehler
PhD student

Phone: + 49 89-3187-49643
E-mail
Building/Room: Campus Neuherberg, building 3620, room 020b

 

 

Project Title

Deciphering the Modulation of Insulin Receptor Signaling during Differentiation of Human iPSCs to β-like Cells

Project description

My project focuses on insulin receptor signaling in human pancreatic beta cell cells during development and adulthood as well as under diabetic conditions. In particular, I am interested in the characterization of two novel modulators of the insulin receptor signaling cascade, which our lab recently discovered. Therefore, I am working with human induced pluripotent stem cells, which can be genetically modified via CRISPR/Cas9 and differentiated towards beta like cells. This in vitro system provides a model of maturing beta cells as well as beta cell development and allows for the translation of findings from mouse models to human cells.

Academic and Research Activities

Since March 2018                       PhD student at Helmholtz Zentrum München, Institute of Diabetes and Regeneration Research, Munich, Germany

March 2017 – March 2018          University of Würzburg, Chair for Drug Formulation and Delivery

Oct. 2013 – Dec. 2016                 Master of Science in Molecular Medicine, University of Freiburg

Oct. 2010 – Aug. 2013                 Bachelor of Science in Molecular Medicine, University of Freiburg

Publications

1. Siehler J*, Blöchinger A*, Lickert H. Pharmacological Targeting of the Actin Cytoskeleton to drive endocrinogenesisTrends in Pharmacological Sciences, April 2020 (* authors contributed equally)

2. Blöchinger A, Siehler J, Wißmiller K, Shahryari A, Burtscher I, Lickert H. Generation of an INSULIN-H2B-Cherry reporter human iPSC lineStem Cell Research, April 2020  

3. Wang, X., Sterr, M., Burtscher, I., Böttcher, A., Beckenbauer, J., Siehler, J., Meitinger, T., Häring, H., Staiger, H., Cernilogar, M., Schotta, G., Irmler, M., Beckers, J., Wright, C., Bakhti, M. & Lickert, H. (2019). Point mutations in the PDX1 transactivation domain impair human β-cell development and function. Molecular metabolism, 24, 80-97.

4. Wang, X., Malinowski, A. R., Beckenbauer, J., Siehler, J., Blöchinger, A., Meitinger, T., Häring, H., Staiger, H., Burtscher, I. & Lickert, H. (2019). Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual. Stem cell research, 39, 101531.