Personal Details

Tobias Greisle
PhD student - Intestinal stem cells

E-mail
Building/Room: Campus Neuherberg, building 3620

Project Title

Dissecting the mechanisms of endocrine lineage formation in gut and pancreas

Project Description

Endocrine cells in the intestine and pancreas secrete various hormones to regulate energy uptake and blood glucose homeostasis. Impairment and dysfunction of these cells is linked to the emergence of obesity, cardiovascular disease and type 2 diabetes, which are leading causes of death worldwide. Understanding the driving factors of endocrinogenesis and identification of conserved pathways is an essential prerequisite for the development of novel therapeutic approaches targeting the regenerative capacity of tissue resident (stem) cells in gut and pancreas. Utilizing mouse models and the potential of CRISPR/Cas9 gene editing tools during in vitro differentiation of human induced pluripotent stem cells (hiPSCs) towards pancreatic and intestinal tissue, we study the role of mechanosignalling and polarity cues during endocrine induction.

Academic and Research Activities

Since Oct. 2020            PhD student, Helmholtz Zentrum München, Institute of Diabetes and Regeneration Research, Neuherberg, Germany

Nov. 2019 – Aug. 2020  Intern/Working student, Roche Diagnostics GmbH, Penzberg, Recombinant Antibody Formats, Dr. Jo Stevens

Apr. 2017 – Mar. 2020  Master of Science in Biology, Technical University Munich, Thesis: “Gene editing of the long non-coding RNA NEAT1 in human embryonic stem cells reveals its role in differentiation.” Institute of Stem Cell Research, Helmholtz Zentrum München, Dr. Micha Drukker

Oct. 2013 – Apr. 2017   Bachelor of Science in Biology, Technical University Munich, Thesis: “Influence of the Th1- and Th2- response on epithelial cells and fibroblasts.” Center of Allergy and Environment Munich, Prof. Dr. Carsten Schmidt-Weber

Publications

Grosch, M., Ittermann, S., Rusha, E., Greisle, T., Ori, C., Truong, D.J., O’Neill, A.C., Pertek, A., Westmeyer, G., Drukker, M. (2020). Nucleus size and DNA accessibility are linked to the regulation of paraspeckle formation in cellular differentiation. BMC Biol. 18, 42.