News

19.09.2020

Publication announcement: DamID to Map Genome-Protein Interactions in Preimplantation Mouse Embryos

The PhD student Mrinmoy Pal published together with Prof. Jop Kind from the Oncode Institute in Utrecht (The Netherlands) and his supervisor Prof. Maria-Elena Torres-Padilla, the Director of the Institute for Epigenetics and Stem Cells, the protocol of "DamID in Preimplantation Mouse Embryos".

Borsos et al., 2019. Nature.

DNA adenine methyltransferase identification (DamID) enables the analysis of genome-wide protein-DNA interactions. In contrast to other protocol, it does not require the use of specific antibodies. Moreover, it can be used to investigate the chromatin landscape in single cells.

The group of researchers describe the application of DamID in single oocytes and mouse preimplantation embryos, as well as single blastomeres, using a Dam-LaminB1 fusion to generate high-resolution lamina-associated domain (LAD) maps. However, this can be adapted for other proteins of interest to faithfully profile their genomic interaction.

References:

Pal M., Kind J., Torres-Padilla M.E. (2020). DamID to Map Genome-Protein Interactions in Preimplantation Mouse Embryos. Methods Mol Biol. PMID: 32944916. DOI: 10.1007/978-1-0716-0958-3_18.