Main Projects

‘Regeneration of the Surface Mesothelium in the Lung & Internal Organs’

project details include:

  • Single cell analysis of the regenerating surface mesothelium
  • Molecular characterization of injured surface mesothelium
  • Target evaluation in mouse models

Schematic illustration of the human coelomic cavities, depicting the surface mesothelium. A single layer of epithelia covers the coelomic cavities on their inner side (parietal surface mesothelia) and the abdominal organs on their outer surface (visceral surface mesothelia), with cobblestone morphology. Source: Rinkevich

‘Stem Cell/Cell Lineage Perspectives of Fibrosis in Lung, Internal Organs & Skin ’

project details include:

  • Identifications of embryonic origins of fibroblasts cell subtypes
  • In vivo functions of fibroblast cell subtypes
  • Live in vivo imaging of fibroblast lineages
  • wound healing models in fetal and adult mice

Photo: Fluorescence histological image taken from En1Cre; R26mTmG double transgenic mice, depicting scar development from a single fibroblast lineage in the dorsal back skin. Source: Rinkevich

‘Stem Cell Lineages in Tissue Regeneration & Tissue Patterning’

project details include:

  • Generation of in vitro organoid assays from single stem cells
  • In vivo clonal analysis & lineage tracing of stem cells during tissue development & regeneration, using ‘Rainbow’ and ‘Tetrachimera’ mouse models
  • Studies in of multi-color ‘Rainbow’ and Tetrachimera’ mouse models
  • Functional studies of gene perturbations in stem cell lineages using mouse models of organ injury

Photo: Fluorescence histological image of the gastrointestinal track from tetrachimera mice. Mice are generated by implanting mouse embryonic stem cells that express separate fluorescent colors (green, red, blue) into wild-type blastocysts. By analyzing tetrachimera mice we are able to determine the lineage compositions of each organ, and follow the cascade of events in lineage appearances during organ development and regeneration that follows injury. Source: Rinkevich