Flow Cytometry / Cell Sorting

The LSRII is a Benchtop Flow Cytometry System with octagon and trigon detector arrays.
It runs with the FACSDiva software version 6.1.3 on a windows machine.

Example of available markers

Emission Filter (nm)

 

Detector

Laser line/color

(Excitation)

PE-Cy7

  780 / 60

  A

  488 nm Blue

  Coherent Sapphire  
  solid-state laser,

  22 mW

PerCP, PerCP-Cy5.5

  695 / 40

  B

PE-Cy5

  660 / 20

  C

PE-Texas Red, PI, 7AAD

  610 / 10

  D

PE

  575 / 26

  E

FITC, Alexa Fluor 488

  530 / 30

  F

SSC

  488 / 10

  G

 

 

 

 

 

APC-Cy7, APC-Alexa 750

  780 / 60

  A

  633 nm Red

  Helium  Neon
  laser, 20 mW

Alexa Fluor 700, Alexa 700

  730 / 45

  B

APC

  660 / 20

  C

 

 

 

 

 

AmCyan, Cascade Yellow ,

Pacific Orange

  525 / 50

  A

  405 nm / Violet

  Coherent VioFlame 
  solid-state laser,

  25 mW

Pacific Blue, Cascade Blue

  450 / 50

  B

 

 

 

 

Indo-1 (blue), PI (UV)

  530 / 30

  A

  355 nm / UV

  Lightwave solid state 
  UV laser with 20 mW
  at 355 nm

Indo-1 (violet), DAPI, Hoechst

  450 / 50

  B