Roche LightCycler 96

The LightCycler 96 Instrument is a real-time PCR system for rapid cycling up to 96 samples. It is an optical detection system that allows flexible detection of sequence-dependent probes, such as hydrolysis probes and sequence-independent dyes (i.e., SYBR Green I). Multiplex and multicolor capabilities permit the use of up to four different fluorescent dyes.


  • Plate-based real-time PCR system with 96-well block
  • Temperature gradient (assay optimization)
  • Dynamic range of 10 log
  • Sensitivity of one copy
  • 4-plex multicolor


Primary applications include :

  • gene expression analysis
  • gene detection
  • genetic variation analysis in mono- or multicolor (up to 4 colors) mode.
  • mutation detection
  • methylation analysis
  • miRNA research
  • relative quantification of target genes.


The software includes analysis modules for:

  • Relative Quantification
  • Absolute Quantification
  • Melting Peak
  • Endpoint Genotyping
  • High Resolution Melting
  • Qualitative Detection