mmRNA

The mmRNA is currently the ultimate method for reprogramming with minimal risk for genetic alternations. The RNA cocktail consists of synthetic mRNAs prepared in vitro and modified with 3’ and 5’ UTR elements that increase their stability. We have optimized the mmRNA reprogramming protocol using POU5F1 (OCT4), LIN28A, KLF4, CMYC and SOX2 for human and primate skin fibroblasts. The reprogramming schedule includes 4 transfection rounds performed daily, and additional 10 days of culturing for generating stable clones iPS cell clones.

Time-lapse imaging of Neonatal Human Dermal Fibroblast cells using Modified mRNA reprogramming