Epigenetic Engineering

Lepko T, Pusch M, Muller T, ...Breunig CT, Stricker SH, Götz M, Ninkovic J. Choriod plexus releases miR-204 regulating the number of neural stem cells in the adult brain. EMBO J. 2019 Jul.

Baumann V, Wiesbeck M, Breunig CT, Braun JM, Köferle A, Ninkovic J, Götz M, Stricker SH. Targeted removal of epigenetic barriers during transcriptional reprogramming. Nature communications. 2019 May.

Singh H, Stricker SH. Glucose regulated TET2 activity links cancer to diabetes.        Trends in Cancer. 2019 Jan.

Breunig CT, Neuner AM, Schwab J, Wurst W, Götz M, Stricker SH. A customizable protocol for string assembly gRNA cloning (STAgR). J.Vis.Exp. 2018 Dec.

Giamo RD, Durovic T, Kociaj A, Lepko T, Breunig CT, .... Wurst W, Stricker SH, Ninkovic J. Aryl hydrocarbon receptor pathway defines the time frame for restorative neurogenesis. Cell Reports. 2018 Dec.

Maher GJ, Ralph HK, ..., Stricker SH, Beck S, McVean G, Wilkie AOM, Goriely A. Selfish mutations dysregulating RAS-MAPK signaling are pervasive in aged human testes. Genome Research. 2018 Dec.

Breunig CT, Durovic T, Neuner AM, Baumann V, Wiesbeck MF, Köferle A, Götz M, Ninkovic J, Stricker SH. One step generation of customizable gRNA vectors for multiplex CRISPR approaches through string assembly gRNA cloning (STAgR).                           PLOS One. 2018 March.

Bultmann S, Stricker SH. Entering the post-epigenomic age - back to epigenetics.     Open Biology. 2018 March.

Stricker SH, Götz M. DNA methylation: Master or slave of neural fate decisions? Frontiers in Neurosciences. 2018 Jan.

Köferle A, Stricker SH. A Universal Protocol for Large-scale gRNA Library Production from any DNA Source. J. Vis. Exp. 2017 Dec.

Stricker SH, Köferle A, Beck S. From profiles to functions in epigenomics.              Nature Reviews Genetics, 2017 Jan.

Köferle A, Worf K, Breunig C, Baumann V, Götz M, Fuchs C, Beck S, Stricker SH. CORALINA: A universal method for the generation of gRNA libraries for CRISPR-based screening. BMC Genomics, 2016 Nov.

Blake SM, Stricker SH, Havalach H, Poetsch AR, Gresswell G, Kelly G, Kanu N, Marino S, Luscombe NM, Pollard SM, Behrends A. Glioblastoma formation induced by loss of p53 requires ATMsignalling. eLIFE, 2016 March.

Caren H, Stricker SH, Bulstrode H, Gagrica S,  Johnstone E, Bartlett TE, Feber E, Wilson G, Teschendorff AE, Bertone E, Beck S, Pollard SM. Glioma stem cells respond to differentiation cues, but fail to undergo commitment and terminal cell-cycle arrest. Stem Cell Reports, 2015 Oct.

Anna Köferle*, Stefan H. Stricker*, Stephan Beck*. Brave new epigenomes: the dawn of epigenetic engineering. Genome Medicine 2015 June. *Corresponding author. Reprint in "The Scientist"

Stricker SH*, Pollard SM*. Reprogramming cancer cells to pluripotency:  An experimental tool for exploring cancer epigenetics. Epigenetics 2014 Mar.   *Corresponding author.

Stefan H. Stricker, Andrew Feber, Pär G. Engström, Helena Carén, Kathreena M. Kurian, Yasuhiro Takashima, Colin Watts, Michael Way, Peter Dirks, Paul Bertone, Austin Smith, Stephan Beck, Steven M. Pollard. Widespread resetting of DNA methylation in glioblastoma initiating cells suppresses malignant cellular behaviour in a lineage dependent manner. Genes and Development 2013 Mar.  Research highlight in Cancer Research (2013 April). Research highlight in Nature Reviews Cancer (2013 May). Recommended by Faculty of 1000.

Danovi D, Folarin A, Ender C, Engstrom P, Gogolok S, Stricker SH, Yu D, Pong UK, Harvey KJ, Ferretti P, Paddison P, Bertone P; Smith A, Pollard SM. A high-content small molecule screen identifies sensitivity of glioblastoma stem cells to inhibition of polo-like kinase. PLOS One 2013 Oct.

Latos AP, Pauler FM, Koerner MV, Senergin BH, Hudson QJ, Stocsits RR, Allhoff W, Stricker SH, Klement RM, Warczok KE, Aumayr K, Pasierbeck P, Barlow DP. Airn transcriptional overlap but not its lncRNA products induce imprinted Igf2r silencing. Science 2012 Dec. Recommended by Faculty of 1000.

Engström PG, Tommei D, Stricker SH, Ender C, Pollard SM and Bertone P, Digital transcriptome profiling of normal and glioblastoma-derived neural stem cells identifies genes associated with patient survival. Genome Medicine 2012 Oct. 

Koerner MV, Pauler FM, Hudson QJ, Santoro F, Sawicka A, Guenzl PM, Stricker SH, Schichl YM, Latos PA, Klement RM, Warczok KE, Wojciechowski J, Seiser C, Kralovics R, Barlow DP. Downstream CpG Island Controls Transcript Initiation and Elongation and the Methylation State of the Imprinted Airn Macro ncRNA Promoter. PLOS Genetics. 2012 Mar.

Pollard SM, Stricker SH, Beck S. A Shore Sign of Reprogramming. Cell Stem Cell. 2009 Dec.

Pollard SM, Yoshikawa K, Clarke ID, Danovi D, Stricker S, Russell R, Bayani J, Head R, Lee M, Bernstein M, Squire JA, Smith A, Dirks P. Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell. 2009 Jun.

Latos AP*, Stricker SH*, Steenpass L* ,Pauler FM, Koerner M, Unger C, Warczok K, Barlow DP. An in vitro ES cell imprinting model shows that imprinted expression of the Igf2r gene arises from an allele-specific expression bias. Development 2009 Feb, *Equal contribution

Stricker SH. Transcription interference as a new model of imprinted gene expression. B.I.F. FUTURA  2009/24

Stricker SH*, Steenpass L*, Pauler FM, Santoro F, Latos PA, Huang R, Koerner MV, Sloane MA, Warczok KE, Barlow DP. Silencing and transcriptional properties of the imprinted Airn ncRNA are independent of the endogenous promoter. EMBO Journal 2008 Dec*Equal contribution. Recommended by Faculty of 1000

Stricker SH, Barlow DP, Latos AP. Size does matter: macro ncRNAs and the regulation of imprinted gene cluster. European Pharmaceutical Review 2008 (1)

Seidl CI*, Stricker SH*, Barlow DP. The imprinted Air ncRNA is an atypical RNAPII transcript that evades splicing and escapes nuclear export. EMBO Journal 2006 Aug 9. *Equal contribution. Recommended by Faculty of 1000

Stricker SH, Meiri K, Gotz M. P-GAP-43 is enriched in horizontal cell divisions throughout rat cortical development. Cerebral Cortex. 2006 Jul.

Gotz M, Stricker SH. Go with the flow: signaling from the ventricle directs neuroblast migration. Nature Neuroscience. 2006 Apr.

Pauler FM, Stricker SH, Warczok KE, Barlow DP. Long-range DNase I hypersensitivity mapping reveals the imprinted Igf2r and Air promoters share cis-regulatory elements. Genome Research. 2005 Oct.

Braidotti G, Baubec T, Pauler F, Seidl C, Smrzka O, Stricker S, Yotova I, Barlow DP. The Air noncoding RNA: an imprinted cis-silencing transcript. Cold Spring Harb Symp Quant Biol. 2004.

Stricker SH, Barlow DP. Significance of repeats in the Air RNA in the imprinting mechanism. B.I.F. FUTURA 2004/19.