Antibody Purification and Labeling

Antibody Purification

We purify antibodies by protein A/G affinity chromatography and gelfiltration. If required, endotoxins are removed.

Antibody elution from the affinity column is performed by a pH shift which may influence antibody stability.  For most applications such as WB, IF, IHC, FACS antibody supernatant works well. We recommend antibody purification only for in vivo experiments and for experiments with directly labelled antibodies.

Antibody Labeling

We conjugate purified monoclonal antibodies to various fluorescendt dyes or enzymes.