Immunization and Fusion

Immunization

Routinely, we immunize two rats and one mouse. The antigen, together with CpG, is emulsified in Freund’s incomplete adjuvant. For peptide immunizations, we perform immunizations with a peptide mixture. The immunization step is followed by a booster injection 6-8 weeks after immunization.

Fusion

Immunized animals are sacrificed 3 days after the final boost injection and splenocytes are collected and prepared for fusion with the mouse myeloma cell line P3X63-Ag8.653. Fused cells are plated in 96-well plates in HAT selection media.