I am interested in generating a monoclonal antibody. Where do I start?

Please get in touch with one of the two scientists in the Monoclonal Antibody Core Facility . We will need as many information of the antigen as possible, i.e.. sequence, structure, localisation within the cell, species, etc) and the intended applications you'd like to use the antibody, i.e. immunoblotting, IHC, IF, Flow Cytometry, or ELISA detection. We also need a project description that explains why the generation of new antibodies against your target of choice is important for your work.

What species will be used to generate my mAbs?

The host selected for your immunization will depend on the type of protein (intra- or extracellular) and the species from which the protein is derived. Usually we immunize two rats and one mouse.

Do I have to validate antibody supernatants?

Yes. You need to tell us which experiments are planned with your antibodies. If you are setting up a new assay, it will be very important to have the assay fully established to allow the screening of the candidate supernatants.

Can I use the supernatants for assays that I have designed?

Yes. For most assays you can directly use the supernatant. Affinity purification is only required for antibody labelling or for life cell experiments. We are adding NaN3 to the supernatant to avoid contamintions.

Does the Core Facility help with the screening process?

Usually we perform a first ELISA screen to identify potential candidate supernatants reacting with your provided antigen. However, depending on the intended assays, we can help with capture, competition or Sandwich ELISA or 96-well format FACS analyses.

How can I find out about the progress of my project?

As you can see from the time line in ‘How we Work’, the generation of mAbs is a long and multi-step procedure. We will contact you once a distinct step is completed and requires your permission to proceed to the next one (for example, after the first ELISA screen). Information on a project can be obtained at any time by contacting the Core Facility.

How do I get billed?

If you are a HMGU researcher, we will request your PSP account. For external partners, we will generate an offer.  Once the offer has been accepted, we will start the project. The production process is divided into three steps, which are billed independently and we will only continue with your approval. We will only charge the steps that have been initiated.

Do you guarantee that I will get an antibody for my project?

Every antibody development project has its risks; one of them is that we cannot guarantee that your peptide or protein will lead to high-affinity antibody production. We have quality control measures in place that will help to increase the chances of success, software programs to analyse antigens and know-how but there are always proteins that fail to produce an antibody despite every prediction. We have experience in generating antibodies, providing every project its best chance for success.

Feel free to contact us if you have further questions.