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Scientific Focus

  • The Protein Expression and Purification Facility (PEPF) was established in the fall of 2009 as part of the Institute of Structural Biology (STB) with the goal to help researchers at the Helmholtz Zentrum München to better and quicker produce the recombinant proteins needed for their research projects. To achieve this goal we take over this part of the projects. We help producing the expression constructs; develop and optimize protocols for the expression and purification of the recombinant proteins; produce these proteins using the optimized protocols; perform biochemical and biophysical characterization of the produced proteins.

    Within the Institute of Structural Biology we are involved in projects linked to among others neurodegeneration and diabetes and projects on DNA and RNA binding proteins.

    In collaboration with researchers from other institutes at the Helmholtz Zentrum München we work on the production of specific proteins for a wide range of applications such as biochemistry, structural biology and chemical biology/drug discovery.

    Furthermore, we produce proteins for general use (such as proteases, polymerases, Cas9, etc.) and provide support and training (among others a HELENA course), materials (such as vectors and strains), as well as facilities for the cultivation of bacteria and insect cells and for the purification and biochemical and biophysical characterization of proteins.