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PEPF Facilities

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Equipment available at the Institute of Structural Biology (building 43b)

Innova 44R shaker/incubator

Bacterial cultivation

  • 3 New Brunswick Scientific Innova 44R shaker/incubator (Eppendorf)

 

Insect and mammalian cell cultivation

  • 2 New Brunswick Scientific Innova 42R shaker/incubator (Eppendorf)
  • HeraCell 150i incubator (Thermo Scientific)
  • New Brunswick Scientific S44i CO2-incubator (Eppendorf)
  • HeraSafe KS laminar flow hood (Thermo Scientific)

     

Microfluidics homogenizer

Cell lysis

  • Sonoplus HD2200 sonicator (Bandelin)
  • M110 homogenizer (Microfluidics)

 

Centrifugation

  • 2 Sorvall Lynx 6000 centrifuge (Thermo Scientific)
  • 2 5810R centrifuge (Eppendorf)

Äkta Purifier

Protein purification

  • 3 Äkta Purifier 10 (GE Healthcare)
  • 1 Äkta Pure (GE Healthcare)

 

Spectroscopy

  • Peqlab Nonodrop 2000 (Thermo Scientific)
  • 2 BioPhotometer plus (Eppendorf)

Viscotek TDA305 detector

Protein characterization

  • Viscotek TDA305 triple array detector (static light scattering) (Malvern Instruments)
  • MicroCal ITC200 MicroCalorimeter (GE Healthcare)
  • DynaPro Nanostar (dynamic light scattering) (Wyatt Technology)
  • Stratagene Mx3005P RT-PCR machine (Agilent Technologies)

 

Other equipment

  • C1000 ThermoCycler (Bio-Rad)
  • Savant SPD 111V SpeedVac concentrator (Thermo Scientific)
  • Micromodulyo Freeze Dryer (Thermo Scientific)