Genetics and Cellular Engineering

2020

Limeng Yang ; Vanessa A N Kraft ; Susanne Pfeiffer ; Juliane Merl-Pham ; Xuanwen Bao* ; Yu An* ; Stefanie M Hauck ; Joel A Schick. Nonsense-mediated decay factor SMG7 sensitizes cells to TNFa-induced apoptosis via CYLD tumor suppressor and the noncoding oncogene Pvt1. Mol Oncol. (doi:10.1002/1878-0261.12754) (2020)

Cacheiro, P.* ; Muñoz-Fuentes, V.* ; Murray, S.A.* ; Dickinson, M.E.* ; Bucan, M.* ; Nutter, L.M.J.* ; Peterson, K.A.* ; Haselimashhadi, H.* ; Flenniken, A.M.* ; Morgan, H.* ; Westerberg, H.* ; Konopka, T.* ; Hsu, C.W.* ; Christiansen, A.V.* ; Lanza, D.G.* ; Beaudet, A.L.* ; Heaney, J.D.* ; Fuchs, H. ; Gailus-Durner, V. ; Sorg, T.* ; Prochazka, J.* ; Novosadova, V.* ; Lelliott, C.J.* ; Wardle-Jones, H.* ; Wells, S.* ; Teboul, L.* ; Cater, H.* ; Stewart, M.* ; Hough, T.* ; Wurst, W. ; Sedlacek, R.* ; Adams, D.J.* ; Seavitt, J.R.* ; Tocchini-Valentini, G.* ; Mammano, F.* ; Braun, R.E.* ; McKerlie, C.* ; Herault, Y.* ; Hrabě de Angelis, M. ; Mallon, A.M.* ; Lloyd, K.C.K.* ; Brown, S.D.M.* ; Parkinson, H.* ; Meehan, T.F.* ; Smedley, D.* ; International Mouse Phenotyping Consortium (Marschall, S. ; Lengger, C. ; Maier, H. ; Seisenberger, C. ; Bürger, A. ; Kühn, R. ; Schick, J. ; Hörlein, A. ; Oritz, O. ; Giesert, F.H.H. ; Beig, J.) Human and mouse essentiality screens as a resource for disease gene discovery. Nat. Commun. 11:655 (2020)

Kraft, V. ; Bezjian, C.T.* ; Pfeiffer, S. ; Ringelstetter, L. ; Müller, C. ; Zandkarimi, F.* ; Merl-Pham, J. ; Bao, X. ; Anastasov, N. ; Kössl, J. ; Brandner, S. ; Daniels, J.D.* ; Schmitt-Kopplin, P. ; Hauck, S.M. ; Stockwell, B.R.* ; Hadian, K. ; Schick, J. GTP cyclohydrolase 1/tetrahydrobiopterin counteract ferroptosis through lipid remodeling. ACS Cent. Sci. 6, 41-53 (2020)

2017

Trümbach D, Pfeiffer S, Poppe M, Scherb H, Doll S, Wurst W, Schick JA. ENCoRE: an efficient software for CRISPR screens identifies new players in extrinsic apoptosis. BMC Genomics. 2017 18:905.

Bowl MR, et al. A large scale hearing loss screen revelas an extensive unexplored genetic landscape for auditory dysfunction. Nat Commun. 2017 Oct 12;8(1):886.

2016

Doll S, Proneth B, Tyurina YY, Panzilius E, Kobayashi S, Ingold I, Irmler M, Beckers J, Aichler M, Walch A, Prokisch H, Trümbach D, Mao G, Qu F, Bayir H, Füllekrug J, Scheel CH, Wurst W, Schick JA, Kagan VE, Angeli JP, Conrad M. ACSL4 dictates ferroptosis sensitivity by shaping cellular lipid composition. Nat Chem Biol. 2016 Nov 4.

Schick JA, Seisenberger C, Beig J, Bürger A, Iyer V, Maier V, Perera S, Rosen B, Skarnes WC, Wurst W. CRISPR-Cas9 enables conditional mutagenesis of challenging loci. Sci Rep. 2016 Sep 1;6:32326.

2015

Rosen B, Schick J, Wurst W. Beyond knockouts: the International Knockout Mouse Consortium delivers modular and evolving tools for investigating mammalian genes. Mamm Genome. 2015 Oct;26(9-10):456-66.

Theodorou M, Rauser B, Zhang J, Prakash N, Wurst W, Schick JA. Limitations of In Vivo Reprogramming to Dopaminergic Neurons via a Tricistronic Strategy. Hum Gene Ther Methods. 2015 Aug;26(4):107-22.

2014

Friedmann Angeli JP, Schneider M, Proneth B, Tyurina YY, Tyurin VA, Hammond VJ, Herbach N, Aichler M, Walch A, Eggenhofer E, Basavarajappa D, Rådmark O, Kobayashi S, Seibt T, Beck H, Neff F, Esposito I, Wanke R, Förster H, Yefremova O, Heinrichmeyer M, Bornkamm GW, Geissler EK, Thomas SB, Stockwell BR, O'Donnell VB, Kagan VE, Schick JA, Conrad M. Inactivation of the ferroptosis regulator Gpx4 triggers acute renal failure in mice. Nat Cell Biol. 2014 Dec;16(12):1180-91.

2013

Andréasson C, Schick AJ, Pfeiffer SM, Sarov M, Stewart F, Wurst W, Schick JA. Direct cloning of isogenic murine DNA in yeast and relevance of isogenicity for targeting in embryonic stem cells. PLoS One. 2013 Sep 13;8(9):e74207.

 Conrad M, Schick J, Angeli JP. Glutathione and thioredoxin dependent systems in neurodegenerative disease: what can be learned from reverse genetics in mice. Neurochem Int. 2013 Apr;62(5):738-49.

Vogel KU, Edelmann SL, Jeltsch KM, Bertossi A, Heger K, Heinz GA, Zöller J, Warth SC, Hoefig KP, Lohs C, Neff F, Kremmer E, Schick J, Repsilber D, Geerlof A, Blum H, Wurst W, Heikenwälder M, Schmidt-Supprian M, Heissmeyer V. Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation. Immunity. 2013 Apr 18;38(4):655-68.

2012

Bradley A, … Schick JZ, … Wurst W. The mammalian gene function resource: the International Knockout Mouse Consortium. Mamm Genome. 2012 Oct;23(9-10):580-6.

2009

Bergmann O, Bhardwaj RD, Bernard S, Zdunek S, Barnabé-Heider F, Walsh S, Zupicich J, Alkass K, Buchholz BA, Druid H, Jovinge S, Frisén J. Evidence for cardiomyocyte renewal in humans. Science. 2009 Apr 3;324(5923):98-102.

2007

Falk A, Karlsson TE, Kurdija S, Frisén J, Zupicich J. High-throughput identification of genes promoting neuron formation and lineage choice in mouse embryonic stem cells. Stem Cells. 2007 Jun;25(6):1539-45.

 

2001

Zupicich J, Brenner SE, Skarnes WC. Computational prediction of membrane-tethered transcription factors. Genome Biol. 2001;2(12):RESEARCH0050.

Mitchell KJ, Pinson KI, Kelly OG, Brennan J, Zupicich J, Scherz P, Leighton PA, Goodrich LV, Lu X, Avery BJ, Tate P, Dill K, Pangilinan E, Wakenight P, Tessier-Lavigne M, Skarnes WC. Functional analysis of secreted and transmembrane proteins critical to mouse development. Nat Genet. 2001 Jul;28(3):241-9.

2000

Paine-Saunders S, Viviano BL, Zupicich J, Skarnes WC, Saunders S. glypican-3 controls cellular responses to Bmp4 in limb patterning and skeletal development. Dev Biol. 2000 Sep 1;225(1):179-87.

Chen H, Bagri A, Zupicich JA, Zou Y, Stoeckli E, Pleasure SJ, Lowenstein DH, Skarnes WC, Chédotal A, Tessier-Lavigne M. Neuropilin-2 regulates the development of selective cranial and sensory nerves and hippocampal mossy fiber projections. Neuron. 2000 Jan;25(1):43-56.